Immobilized Artificial Membrane Chromatography: Surface Chemistry and Applications
Immobilized Artificial Membranes are solid surfaces containing phospholipids immobilized on silica particles at surface densities similar to the ligand density of reversed phase Chromatographic surfaces. Chromatographic and non-chromatographic applications of Immobilized Artificial Membrane surfaces are reviewed and compared to the Chromatographie and non-chromatographic applications of reversed phase columns. The methodology for synthesizing Immobilized Artificial Membranes and the stability of Immobilized Artificial Membranes are also described. Several examples are presented regarding the ability of Immobilized Artificial Membrane surfaces to model biological processes. Examples include predicting the transport of solutes across human skin, predicting the transport of amino acids across the blood brain barrier, and predicting the binding of solutes to liposome membranes. In addition, the purification of several membrane proteins, including cytochrome P450 from rat adrenals and rat livers, NADH oxidase, and rabbit intestinal phospholipid binding protein, are discussed.
KeywordsHigh Pressure Liquid Chromatography Reversed Phase Column Packing Material Artificial Membrane Propionic Anhydride
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