Lymphocyte Activation Induced Apoptosis by the APO-1 Cell Surface Receptor
Growth of lymphocytes is controlled by cell surface molecules and their ligands including cytokines. To characterize cell surface molecules involved in control of growth of malignant lymphocytes we raised monoclonal antibodies against the human B lymphoblast cell line SKW6.4. One antibody, anti-APO-1, reacted with a 48-kilodalton antigen (APO-1) on a set of activated human lymphocytes, on malignant human lymphocyte lines, and on a fraction of leukemic cells from patients. Nanogram quantities of anti-APO-1 completely blocked proliferation of APO-1 positive cells in vitro in a manner characteristic of a process called programmed cell death or apoptosis . Cell death was preceded by changes in cell morphology and fragmentation of DNA. This process was distinct from antibody and complement dependent cell lysis and mediated by the antibody alone1. In the following, results obtained in this experimental system are discussed in more detail.
KeywordsFollicular Lymphoma Phosphoglycerate Mutase Thymic Selection Follicle Center Follicle Center Cell
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- 4.A. Oehm, I. Behrmann, W. Falk, G. Maier, C. Klas, M. Li-Weber, M. Pawlita, S. Richards, J. Dhein, B.C. Trauth and P.H. Krammer. Purification and molecular cloning of the APO-1 antigen, a new member of the TNF/NGF receptor superfamily: sequence identity with the Fas antigen. J Biol Chem 267: 10709 (1992).PubMedGoogle Scholar
- 7.F. Leithäuser, J. Dhein, G. Mechtersheimer, K. Koretz, S. Brüderlein, C. Henne, A. Schmidt, K.-M. Debatin, P.H. Krammer and P. Möller. Constitutive and induced expression of APO-1, new member of the NGF/TNF receptor superfamily, in normal and neoplastic cells. Lab Invest. In press.Google Scholar
- 13.P. Möller, C. Henne, F. Leithäuser, A. Eichelmann, A. Schmidt, S. Brüderlein, J. Dhein and P.H. Krammer. Co-regulation of the APO-1 antigen with ICAM-1 (CD54) in tonsillar B cells and coordinate expression in follicular center B cells, in follicle center and mediastinal B cell lymphomas. Blood 81: 2067 (1993).PubMedGoogle Scholar