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Apoptosis pp 237-248 | Cite as

Lymphocyte Activation Induced Apoptosis by the APO-1 Cell Surface Receptor

  • Peter H. Krammer
Part of the Pezcoller Foundation Symposia book series (PFSO, volume 5)

Abstract

Growth of lymphocytes is controlled by cell surface molecules and their ligands including cytokines. To characterize cell surface molecules involved in control of growth of malignant lymphocytes we raised monoclonal antibodies against the human B lymphoblast cell line SKW6.4. One antibody, anti-APO-1, reacted with a 48-kilodalton antigen (APO-1) on a set of activated human lymphocytes, on malignant human lymphocyte lines, and on a fraction of leukemic cells from patients. Nanogram quantities of anti-APO-1 completely blocked proliferation of APO-1 positive cells in vitro in a manner characteristic of a process called programmed cell death or apoptosis . Cell death was preceded by changes in cell morphology and fragmentation of DNA. This process was distinct from antibody and complement dependent cell lysis and mediated by the antibody alone1. In the following, results obtained in this experimental system are discussed in more detail.

Keywords

Follicular Lymphoma Phosphoglycerate Mutase Thymic Selection Follicle Center Follicle Center Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    B.C. Trauth, C. Klas, A.M.J. Peters, S. Matzku, P. Möller, W. Falk, K.-M. Debatin and P.H. Krammer. Monoclonal antibody-mediated tumor regression by induction of apoptosis. Science 245: 301 (1989).PubMedCrossRefGoogle Scholar
  2. 2.
    H.-R. Köhler, J. Dhein, G. Alberti and P.H. Krammer. Ultrastructural analysis of apoptosis by the monoclonal antibody anti-APO-1 on a lymphoblastoid B cell line (SKW6.4). Ultrastructural Pathology 14: 513 (1990).PubMedCrossRefGoogle Scholar
  3. 3.
    N. Itoh, S. Yonehara, A. Ishii, M. Yonehara, S-I. Mizushima, M. Sameshima, A. Hase, Y. Seto, and S. Nagata. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell 66: 223 (1991).CrossRefGoogle Scholar
  4. 4.
    A. Oehm, I. Behrmann, W. Falk, G. Maier, C. Klas, M. Li-Weber, M. Pawlita, S. Richards, J. Dhein, B.C. Trauth and P.H. Krammer. Purification and molecular cloning of the APO-1 antigen, a new member of the TNF/NGF receptor superfamily: sequence identity with the Fas antigen. J Biol Chem 267: 10709 (1992).PubMedGoogle Scholar
  5. 5.
    P. Lichter, H. Walczak, S. Weitz, I. Behrmann, and P. H. Krammer. The human APO-1 antigen maps to 10q23, a region which is syntenic with mouse chromosome 19. Genomics 14: 179 (1992).PubMedCrossRefGoogle Scholar
  6. 6.
    R. Watanabe-Fukunaga, C.I. Brannan, N. Itoh, S. Yonehara, N.G. Copeland, N.A. Jenkins and S. Nagata. The cDNA structure, expression and chromosomal assignment of the mouse Fas antigen. J Immunol 148: 1274 (1992).PubMedGoogle Scholar
  7. 7.
    F. Leithäuser, J. Dhein, G. Mechtersheimer, K. Koretz, S. Brüderlein, C. Henne, A. Schmidt, K.-M. Debatin, P.H. Krammer and P. Möller. Constitutive and induced expression of APO-1, new member of the NGF/TNF receptor superfamily, in normal and neoplastic cells. Lab Invest. In press.Google Scholar
  8. 8.
    R. Watanabe-Fukunaga, C.I. Brannan, N.G. Copeland, N.A. Jenkins, and S. Nagata, Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis. Nature 356: 314 (1992).PubMedCrossRefGoogle Scholar
  9. 9.
    C.L. Sidmann; J.D. Marshall, and H. von Boehmer. Transgenic T cell receptor interactions in the lymphoproliferative and autoimmune syndromes of lpr and gld mutant mice. Eur J Immunol 22: 499 (1992).CrossRefGoogle Scholar
  10. 10.
    P.L. Cohen and R.A. Eisenberg. The lpr and gld genes in systemic autoimmunity: life and death in the Fas lane. Immunol Today 13: 427 (1992).PubMedCrossRefGoogle Scholar
  11. 11.
    P.H. Krammer and K.-M. Debatin. When apoptosis fails. Curr Biol 2: 383 (1992).PubMedCrossRefGoogle Scholar
  12. 12.
    C. Klas, K.-M. Debatin, R.R. Jonker and P.H. Krammer. Activation interferes with the APO-1 pathway in mature human T cells. Internatl Immunol. 5: 625 (1993).CrossRefGoogle Scholar
  13. 13.
    P. Möller, C. Henne, F. Leithäuser, A. Eichelmann, A. Schmidt, S. Brüderlein, J. Dhein and P.H. Krammer. Co-regulation of the APO-1 antigen with ICAM-1 (CD54) in tonsillar B cells and coordinate expression in follicular center B cells, in follicle center and mediastinal B cell lymphomas. Blood 81: 2067 (1993).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Peter H. Krammer
    • 1
  1. 1.Division of ImmunogeneticsGerman Cancer Research CenterHeidelbergGermany

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