Non-Traditional Approaches for Quality Control of Antimicrobial Susceptibility Tests
Quality control is an essential part of any clinical laboratory procedure and can simply be defined as the series of steps that are taken to make certain test results are accurate and reproducible. Antimicrobial susceptibility tests, like many other microbiology tests are somewhat unique in that they depend on growth (or no growth) of bacteria which is often unpredictable and sensitive to changes in the environment. Therefore, it is essential that technical variables in the test system are well standardized and controlled and that personnel performing the tests thoroughly understand the components of the test system. This must all be considered when developing a program for quality control of antimicrobial susceptibility tests.
KeywordsInfective Endocarditis Antimicrobial Susceptibility Clinical Laboratory Standard Clinical Microbiology Laboratory Viridans Streptococcus
Unable to display preview. Download preview PDF.
- 1.American Type Culture Collection (ATCC). 1989. Catalogue of bacteria and bacteriophages. 17th edition. ATCC, Rockville, MD. (301) 881–2600.Google Scholar
- 4.A.L. Bisno, WE Dismukes, DT Durack, E.L. Kaplan, A.W. Karchmer, D. Kaye, S.H. Rahimtoola, M.A. Sande, J.P. Sanford, C. Watanakunakorn, and W.W. Wilson, Antimicrobial treatment of infective endocarditis due to viridans streptococci, enterococci, and staphylococci. JAMA 261:1471–1477 (1989).PubMedCrossRefGoogle Scholar
- 5.College of American Pathologists (CAP) Proficiency Survey, Bacteriology Survey #D-05. CAP, Skokie, IL. (1991).Google Scholar
- 7.CDC Guidelines for Isolation Precautions in Hospitals, Nosocomial Infections, Infection Control 4:245–249 (1983).Google Scholar
- 8.G.L. Gilardi, Identification of glucose-nonfermenting gram-negative rods, Department of Laboratories, North General Hospital, 1919 Madison Avenue, New York, NY. 10035. (1990).Google Scholar
- 10.G.A. Jacoby, and G.L. Archer, New mechanisms of bacterial resistance to antimicrobial agents. NEJM. 324:601–612 (1990).Google Scholar
- 13.Medical Letter, The choice of antimicrobial agents, The Medical Letter. 32:41–48 (1990).Google Scholar
- 16.National Committee for Clinical Laboratory Standards, 1990, Performance standards for antimicrobial disk susceptibility tests — Fourth edition. Approved Standard; M2–A4. NCCLS, Villanova, PA.Google Scholar
- 17.National Committee for Clinical Laboratory Standards, 1990, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically — Second edition. Approved Standard: M7–A2. NCCLS, Villanova, PA.Google Scholar
- 18.National Committee for Clinical Laboratory Standards, 1991, Supplemental table for M2–A4, M7–A2, and M11–A2, M100–S3, NCCLS, Villanova, PA.Google Scholar
- 21.A. Von Graevenitz, Use of antimicrobial agents as tools in epidemiology, identification, and selection of microorganisms, p. 723–738. In V Lorian (ed.), Antibiotics in laboratory medicine, third edition. Williams & Wilkins, Baltimore (1991).Google Scholar