Advertisement

An Improved Method for in Vitro Susceptibility Testing of Trichomonas Vaginalis

  • Bruno J. Bromke
  • Merewyn C. Furiga
  • Russell C. Hendershot
  • Michelle McGinn
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 390)

Abstract

In vitro susceptibility testing of Trichomonas vaginalis has been performed by many authors.1–10 Most have adapted the classical broth macrodilution methodology to test tubes or microwell plates. However, Lossick11 has criticized susceptibility testing of T. vaginalis for its failure to predict treatment outcome in patients and for lack of standardized practical procedures. For example, some investigators employed an incubation time period that did not correlate with the growth curve of the protozoan,4,10 had no negative growth control,1–10 did not use a standardized inoculum8,10 and/or required an additional subculturing for determination of Minimum Lethal Concentration (MLC). 2,3,5,6,9,10

Keywords

Susceptibility Testing Initial Cell Density Predict Treatment Outcome Lipid Supplement TRICHOMONAS VAGINALIS 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    H.L. Howes, J. E. Lynch, and J. L. Kivlin. 1970. Tinidazole, a new antiprotozoal agent: effect on Trichomonas and other protozoa. Antimicrob. Agents Chemother. 1969: 261–266.Google Scholar
  2. 2.
    R. Nielsen. 1973. Trichomonas vaginalis II. Laboratory investigations in trichomoniasis. Brit. J. Vener. Dis. 49: 531–535.Google Scholar
  3. 3.
    B. Korner and H. K. Jensen. 1976. Sensitivity of Trichomonas uaginalis to metronidazole, tinidazole and nifuratel in vitro. Brit. J. Vener. Dis. 52: 404–408.Google Scholar
  4. 4.
    R.F. Smith and A. Di Domenico. 1980. Measuring the in vitro susceptibility of Trichomonas vaginalis to metronidazole. A disk broth method. Sex. Transm. Dis. 7: 120–124.Google Scholar
  5. 5.
    E.D. Ralph, R. Darwish, T. W. Austin, E. A. Smith, and F. L. M. Pattison. 1983. Susceptibility of Trichomonas vaginalis strains to metronidazole: response to treatment. Sex. Transm. Dis. 10: 119–122.Google Scholar
  6. 6.
    J.N. Krieger, C. S. Dickins, and M. F. Rein. 1985. Use of a time-kill technique for susceptibility testing of Trichomonas uaginalis. Antimicrob. Agents Chemother. 27: 332–336.Google Scholar
  7. 7.
    J.A. Escario, A. M. Igea, M. Contreras, A. R. Martinez-Fernandez, R. Claramunt, and C. Lopez. 1988. Antiparasitic activity of nine pyrazole derivatives against Trichomonas uaginalis, Entamoeba invaders, and Plasmodium berghei. Ann. Trop. Med. Parasitol. 32: 257–262.Google Scholar
  8. 8.
    W.H.R. Lumsden, C. Harrison, and D. H. H. Robertson. 1988. Treatment failure of Trichomonas uaginalis infections in females. II. In vitro estimation of the sensitivity of the organism to metronidazole. J. Antimicrob. Chemother. 21: 555564.Google Scholar
  9. 9.
    M.J. Muller, G. Lossick, and T. E. Gorrell. 1988. In vitro susceptibility of Trichomonas vaginalis to metronidazole and treatment outcome in vaginal trichomoniasis. Sex. Transco. Dis. 15: 17–24.Google Scholar
  10. 10.
    S.D. Sears and J. O’Hare. 1988. In vitro susceptibility of Trichomonas vaginalis to 50 antimicrobial agents. Antimicrob. Agents Chemother. 32: 144–146.Google Scholar
  11. 11.
    J.G. Lossick. 1990. Therapy of urogenital trichomoniasis, p. 324–341. In B. M. Honigberg (ed.), Trichomonads parasitic in humans. Springer-Verlag, New York.CrossRefGoogle Scholar
  12. 12.
    B.J. Bromke and M. Furiga. 1991. Carrageenan is a desirable substitute for agar in media growing Trichomonas uaginalis. J. Microb. Methods 13: 61–65.Google Scholar
  13. 13.
    B.J. Bromke. 1986. A serum-free, lipid-supplemented medium for the growth of Trichomonas uaginalis. J. Microb. Methods 6: 55–59.Google Scholar
  14. 14.
    G.E. Garber and L. T. Lemchuk-Favel. 1989. Characterization and purification of extracellular proteases of Trichomonas vaginalis. Can. J. Microbiol. 35: 903–909.Google Scholar
  15. 15.
    G. Wagner and R. Levin. 1978. Oxygen tension of the vaginal surface during sexual stimulation in the human. Fert. Steril. 30: 50–53.Google Scholar
  16. 16.
    D.L. Linstead. 1990. Cultivation, p. 91–111. In B. M. Honigberg (ed.), Trichomonads parasitic in humans. Springer-Verlag, New York.CrossRefGoogle Scholar
  17. 17.
    D.H. Robertson, R. Heyworth, C. Harrison and W. H. R. Lumsden. 1988. Treatment failure in Trichomonas uaginalis infections in females. I. Concentrations of metronidazole in plasma and vaginal content during normal and high dosage. J. Antimicrob. Chemother. 21: 373–378.Google Scholar
  18. 18.
    J.A. McFadzean, I. M. Pugh, S. L. Squires, and J. P. F. Whelan. 1969. Further observations on strain sensitivity of Trichomonas vaginalis to metronidazole. Brit. J. Vener. Dis. 45: 161–162.Google Scholar
  19. 19.
    P. Sithithaworn, J. Sithithaworn, K. Kitjaroen, C. Pairojkul, S. Ratananurakpong, and T. Sriswangwonk. 1989. Comparative in vitro sensitivity to metronidazole of Trichomonas vaginalis with isolates and clones. Southeast Asian J. Trop. Med. Publ. H. 20: 279–284.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Bruno J. Bromke
    • 1
  • Merewyn C. Furiga
    • 1
  • Russell C. Hendershot
    • 1
  • Michelle McGinn
    • 1
  1. 1.Department of Microbiology and ImmunologyPhiladelphia College of Osteopathic MedicinePhiladelphiaUSA

Personalised recommendations