Advertisement

Angiogenesis pp 143-147 | Cite as

Release of Gangliosides in the Microenvironment and Angiogenic Response in Vivo

  • Pietro M. Gullino
Chapter
Part of the NATO ASI Series book series (NSSA, volume 263)

Abstract

This is a summary of results obtained in experiments stimulated by the following observation: melanosarcoma cells injected in the center of a rabbit cornea migrate toward the periphery and as soon as they reach a distance of 1.5–2.0 mm from the limbus a large number of microvessels develop to colonize the neoplastic cell population. Thereafter, rapid growth of a solid tumor ensues. The objective of our experiments was to analyse the mechanism of this event on the assumption that by blocking angiogenesis the formation of a solid tumor could be avoided or impaired. The experimental approach was based on the following hypothesis: In the adult organism angiogenesis is a multifactorial event: the analysis of tissue composition at the onset of an angiogenic response may suggest which events have determinant infuence on the process.

Keywords

Sialic Acid Angiogenic Response Microvascular Endothelium Rabbit Cornea Angiogenic Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    R. Langer and J. Folkman, Polymers for the sustained release of proteins and other macromolecules, Nature (London) 263: 797 (1976).CrossRefGoogle Scholar
  2. 2.
    G. Alessandri, K.S. Raju, and P.M. Gullino, Interaction of ganglio-sides with fibronectin in the mobilization of capillary endothelium,Invasion and Metastasis 6: 145 (1986).Google Scholar
  3. 3.
    G. Alessandri, S. Flippeschi, P. Sinibaldi, F. Mornet, P. Passera, F. Spreafico, A.P.M. Cappa, and P.M. Gullino PM, Influence of gangliosides on primary and metastatic neoplastic growth in human murine cells, Cancer Res. 47: 4243 (1987).PubMedGoogle Scholar
  4. 4.
    G. Yogeeswaran, and P.L. Salk, Metastatic potential is positively correlated with cell surface sialylation of cultured murine tumor cell linen Science (Wash DC) 212: 1514 (1981).Google Scholar
  5. 5.
    M. Ziche, G. Alessandri, and P.M. Gullino, Gangliosides promote the angiogenic response, Lab. Invest. 61: 629 (1989).Google Scholar
  6. 6.
    G. Alessandri, G. De Cristan, M. Ziche, A.P.M. Cappa, and P.M. Gullino, Growth and motility of microvascular endothelium are modulated by the relative concentration of gangliosides in the medium, J. Cell Physiol. 151: 23 (1992).PubMedCrossRefGoogle Scholar
  7. 7.
    G.De Cristan, L. Morbidelli, G. Alessandri, M. Ziche, A.P.M. Cappa, and P.M. Gullino, Synergism between gangliosides and basic fibroblastic growth factor in favouring survival, growth and motility of capillary endothelium, J. Cell Physiol. 144: 505 (1990).Google Scholar
  8. 8.
    M. Ziehe, L. Morbidelli, G. Alessandri, P.M. Gullino, Angiogenesis can be stimulated or repressed in vivo by a change in GM3:GD3 ganglioside ratio, Lab. Invest. 67: 711 (1992).Google Scholar
  9. 9.
    M. Ziche, and P.M. Gullino, Angiogenesis and neoplastic progression in vitro, J. Nat. Cancer Inst. 69: 483 (1982).PubMedGoogle Scholar
  10. 10.
    S.S. Brem, H. Jensen, and P.M. Gullino, Angiogenesis as a marker of preneoplastic lesions of the human breast, Cancer 41: 239 (1978).PubMedCrossRefGoogle Scholar
  11. 11.
    M. Ziche, J. Jones, and P.M. Gullino, Role of prostaglandin El and copper in angiogenesis, J. Nat. Cancer Inst. 69: 475 (1982).PubMedGoogle Scholar
  12. 12.
    J. Kandel, E. Bossy-Wetzel, I.F. Radvany, M. Klagbrun, and J.Folkman, Neovascularization is associated with a switch to the export of bFGF in the multi-step development of fibrosarcoma, Cell 66: 1095 (1991)PubMedCrossRefGoogle Scholar
  13. 13.
    M.H. Ravindranath, T. Tsuchida, and R.F. Irie, Diversity of ganglioside expression in human melanoma, in: “Gangliosides and Cancer,” H.F. Oettgen, ed., VCF, p. 81, New York (1989).Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Pietro M. Gullino
    • 1
  1. 1.Department of Biomedical SciencesUniversity of TorinoTorinoItaly

Personalised recommendations