Patterns of Physiological Angiogenesis in Adult Mesentery

  • F. Hansen-Smith
  • Laura Morris
Part of the NATO ASI Series book series (NSSA, volume 298)


The structural process by which new microvessels form from pre-existing ones has been documented in classical studies of wound healing. (Folkman and Shing, 1992; Hudlicka and Tyler, 1986). However, the process by which new microvessels originate de novoand continue to expand under physiological conditions is less well understood. The physiological formation and subsequent growth of a new vascular bed is normally considered to be something that occurs predominantly during embryonic development. In mammalian systems this puts certain restrictions on the types of studies which can be done on a developing vascular bed to understand how the growth is regulated. Most tissues in the adult have little ongoing angiogenesis, with the exception of the female reproductive system. However, it has been found that the rat mesentery undergoes a spontaneous angiogenesis, leading to the formation of a two-dimensional microvascular bed which seems to be well suited for studies of angiogenesis under either physiological or pathological conditions (Hansen-Smith, Joswiak, and Baustert, 1994; Norrby, Jakobsson, and Sorbo, 1990; Rhodin and Fujita, 1989). Since this growth occurs in adults, many types of physiological studies, as well as structural and biochemical studies, are feasible to help understand not only how the initiation of angiogenesis and angiostasis are regulated, but also how microvascular patterns arise and are remodelled during the enlargement of microvascular networks.


Mast Cell Female Reproductive System Microvascular Network Portal Side Capillary Plexus 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Colville-Nash, P.R. and Wiloughby, D.A., 1997, Growth factors in angiogeneis: current interest and therapeutic potential. Mol. Med. Today, 14–23.Google Scholar
  2. D’Amore, P., 1992, Mechanisms of endothelial growth control, Am. J. Resp. Cell. Mol. Biol. 6: 1–8.CrossRefGoogle Scholar
  3. D’Amore, P., and Smith, S.R., 1993, Growth factor effects on cells of the vascular wall: a survey. Growth Factors 8: 61–75, 1993.PubMedCrossRefGoogle Scholar
  4. Folkman, J. and Shing, Y., 1992, Angiogenesis. J. Biol. Chem. 267: 10931–34.PubMedGoogle Scholar
  5. Hansen-Smith, F.M., 1988, Fluorescent delineation of microvessels in thin muscle preparations and mesentery. FASEB J. 2, A1189Google Scholar
  6. Hansen-Smith, F.M., Watson, L., Du, D., and Goldstein, I., 1988, Griffonia simplicifolia I: Fluorescent tracer for microcirculatory vessels in non-perfused thin muscles and sectioned muscle. Microvasc. Res. 36: 199–215.PubMedCrossRefGoogle Scholar
  7. Heuser, L., Taylor, S., Folkman, J., 1984, Prevention of carcinomatosis and blood malignant ascites in the rat by an inhibitor of angiogenesis. J. Surg. Res. 36: 244–50.PubMedCrossRefGoogle Scholar
  8. Hudlicka, O., and Tyler, K.R. Angiogenesis. New York, Academic Press, 1986.Google Scholar
  9. Jakobsson, A., 1994, Angiogenesis induced by mast cell secretion in rat peritoneal connective tissue is a process of three phases. Microvasc. Res. 47: 252–69.PubMedCrossRefGoogle Scholar
  10. Nagy, J., Morgan, E., Herzberg, K., Manseau, E., Dvorak, A., and Dvorak, H., 1995, Pathogenesis of ascites tumor growth: angiogenesis, vascular remodeling, and stroma formation in the peritoneal lining. Cancer Res. 55: 376–85.PubMedGoogle Scholar
  11. Nehls, V. and Drenckhahn, D., 1991, Heterogeneity of microvascular pericytes for smooth muscle type alpha-actin. J. Cell Biol 113: 147–54.PubMedCrossRefGoogle Scholar
  12. Norrby, K. and Sorbo, J., 1992, Heparin enhances angiogenesis by a systemic mode of action. Int. J. Exp. Pathol. 73: 147–155.PubMedGoogle Scholar
  13. Norrby, K., Jakobsson, A., and Sorbo, J., 1986, Mast-cell mediated angiogenesis: a novel experimental model using the rat mesetery. Virchow’s Arch. B. Cell Pathol. 52: 195–206.CrossRefGoogle Scholar
  14. Norrby, K., Jakobsson, A., and Sorbo, J., 1989, Mast-cell secretion and angiogenesis: a quantitative study in rats and mice. Virchows. Arch. 57: 251–56.CrossRefGoogle Scholar
  15. Norrby, K., Jakobsson, A., and Sorbo, J., 1990, Quantitative angiogenesis in spreads of intact rat mesenteric windows. Microvasc. Res. 39: 341–348.PubMedCrossRefGoogle Scholar
  16. Rhodin, J. A. G. and Fujita, H., 1989, Capillary growth in the mesentery of normal young rats: Intravital video and electron microscope analyses. J. Submicrosc. Cytol. Pathol. 21: 1–34.PubMedGoogle Scholar
  17. Sorbo, J., Jakobsson, A., and Norrby, K., 1994, Mast-cell histamine is angiogenic through receptors for histamine1 and histamine2. Int. J. Exp. Path. 75: 43–50.Google Scholar
  18. Unthank, J., Lash, J., and Bohlen, H., 1990, Maturation of the rat intestinal microvasculature from juvenile to early adult life. Am. J. Physiol. 259: G282–289.Google Scholar
  19. Yanagi, K. and Oshima, N., 1996, Angiogenic vascular growth in the rat peritoneal disseminated tumor model. Microvasc. Res. 51: 15–28.PubMedCrossRefGoogle Scholar
  20. Zweifach, B., 1973, The microcirculation in the intestinal mesentery. Microvasc. Res. 5: 363–367.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • F. Hansen-Smith
    • 1
  • Laura Morris
    • 1
  1. 1.Department of Biological SciencesOakland UniversityRochesterUSA

Personalised recommendations