Lactoferrin, Anti-Lactoferrin Antibodies and Inflammatory Disease
The nucleophilic properties of human lactoferrrin (Lf) were demonstrated by immunofluorescence microscopy using cryostat rat tissue sections, and the nuclear/perinuclear distribution of Lf in ethanol-fixed human neutrophils was visualized with rabbit anti-human Lf, producing a P-ANCA/GS-ANA staining pattern. Prevention of complement activation by Lf was confirmed in a haemolytic assay. Antibodies (IgG) against human Lf were studied by ELISA in sera from patients with Crohn’s disease, ulcerative colitis, primary sclerosing cholangitis, rheumatoid arthritis, systemic lupus erythematous and primary Sjögren’s syndrome. Anti-Lf antibodies were found in high frequency in ulcerative colitis and primary sclerosing cholangitis, but only occasionally in the other conditions.
KeywordsSystemic Lupus Erythematosus Inflammatory Bowel Disease Ulcerative Colitis Primary Sclerosing Cholangitis Sjogrens Syndrome
Unable to display preview. Download preview PDF.
- Birgens H. The interaction of lactoferrin with human monocytes. (Review) Dan.Med. Bull. 1991; 38: 244252Google Scholar
- Coremans IEM, Hagen EC, van der Woude FJ et al (1992): Anti-lactoferrin antibodies in patients with rheumatoid arthritis with vasculitis. Clin.Rheumatol. 11: 4Google Scholar
- Esaguy N, Aguas AP, van Embden JDA, Silva MT (1991): Mycobacteria and human autoimmune disease: direct evidence for cross-reactivity between human lactoferrin and the 65-kilodalton protein of tubercle and leprosy bacilli. Inf.Immun. 59: 1117–25Google Scholar
- Goldschmeding R. Target antigens of antineutrophil cytoplasmic antibodies. Thesis (MD), Amsterdam 1992Google Scholar
- Kijlstra A. The role of lactoferrin in the nonspecific immune response on the occular surface. Reg.Immunol. 1990–91; 3: 193–97Google Scholar
- Skogh T, Dahlgren C, Holmgren K, Peen E, Stendahl 0 (1991): Anti-granulocyte anti-bodies (C-ANCA, P-ANCA, GS-ANA) studied by confocal scanning laser fluorescence microscopy, ELISA, and chemiluminescence techniques. Scand J Immunol. 34: 137–45Google Scholar