Anti-Neutrophil Cytoplasmic Antibodies in Giant Cell Arteritis and Polymyalgia Rheumatica
Antineutrophil cytoplasmic antibodies (ANCA) were demonstrated in all sera from 11 patients with active giant cell arteritis (GCA) using indirect immunofluorescence on 9% paraformaldehyde(PF)-fixed neutrophils according to Pryzwanski (median titer 1:256, range 1:64 to 1:512). After treatment during inactive disease titers decreased in all sera. Eight out of 9 sera from patients with active polymyalgia rheumatica (PMR) produced a cytoplasmic staining on Pryzwanski-fixed neutrophils in low titers (median titer 1:16, range 0 to 1:32), as did 8 out of 25 sera from healthy blood donors. None of the sera were positive for antibodies to defined antigens, i.e. proteinase-3, human leucocyte elastase, myeloperoxidase and lactoferrin as detected by ELISA. GCA seems to be associated with ANCA of as yet unknown specificity.
KeywordsArthritis Corticosteroid Titration Sedimentation Peri
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- 1.Kallenberg, C.G.M., and Cohen Tervaert, J.W., 1992, Anti-neutrophil cytoplasmic antibodies (ANCA): new tools in the diagnosis and follow-up of necrotizing glomerulonephritis and vasculitis, in: “International Yearbook of Nephrology”, V.E. Andreucci, and L.G. Fine, eds, Springer Verlag, London.Google Scholar
- 2.Charles, L.A., Falk, R.J., and Jennette, J.C, 1989, Reactivity of anti-neutrophil cytoplasmic autoantibodies with HL-60 cells, Clin. Immunol. Immunopathol. 53: 243-253.Google Scholar
- 3.Cohen Tervaert, J.W., Brouwer, E., Mulder, A.H.L., Limburg, P.C., and Kallenberg, C.G.M., 1991, The detection of myeloperoxidase antibodies: a comparison of four different techniques (abstract), Am. J. Kidney Dis. 18: 198.Google Scholar
- 5.Jones, J.G., and Hazleman, B.L., 1981, Prognosis and management of polymyalgia rheumatica, Ann. Rheum. Dis. 40: 1-5.Google Scholar
- 6.Weinick, R., LoSpalluto, J.J., Fink, C.W., and Ziff, M.Y., 1981, Serum IgG and IgM rheumatoid factors by solid phase radioimmunoassay. A comparison between adult and juvenile rheumatoid arthritis, Arthritis Rheum. 24: 1501-1511.Google Scholar