Methods of Detection of Anticathepsin G Autoantibodies in Human
Anticathepsin G antibodies have been detected by using three different methods. i) Binding to azurophilic granules constituants after separation of purified α-granules on Matrex gel Orange A chromatography according to Kao. ii) Binding to azurophilic granules freezed and thawed after coating on ELISA plates. iii) Binding to purified cathepsin G in ELISA assay.
Anticathepsin G antibodies were observed patient’s sera with ulcerative colitis, primary sclerosing cholangitis, primary biliary cirrhosis and autoimmune hepatitis but not in controls or patients with chronic viral hepatitis or vasculitis.
KeywordsUlcerative Colitis Primary Biliary Cirrhosis Primary Sclerosing Cholangitis Autoimmune Hepatitis Chronic Viral Hepatitis
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