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In Vitro Interactions of C-ANCA (Antibodies to Proteinase 3) with Human Endothelial Cells

  • Werner-J. Mayet
  • Elisabeth M. Hermann
  • Elena Csernok
  • Wolfgang L. Gross
  • Karl-H. Meyer zum Büschenfelde
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 336)

Abstract

Several concepts concerning the pathogenicity of antineutrophil cytoplasmic antibodies (ANCA) exist, but till now only sparse data about ANCA-endothelial interactions are available. In this study we have investigated the expression of proteinase 3 (PR-3) in human umbilical endothelial cells (HEC) using purified anti-PR3 antibodies (C-ANCA) of patients with Wegener’s granulomatosis (WG) and monoclonal antibodies to PR-3 (human and murine) as probes. Performing cytoELISAs, laser scanning microscopy and Western blot we were able to show that treatment of HEC with IL-1-alpha led to an increased PR-3 expression in the cytoplasm and to a transient translocation into the EC-membrane. Representing an important missing link of ANCA-endothelial interactions, our data give a hint at a possible direct pathogenicity of anti-PR-3 antibodies in WG and other vasculitides.

Keywords

Human Endothelial Cell Systemic Vasculitis Antineutrophil Cytoplasmic Antibody Human Umbilical Cord Endothelial Cell Original Magnification 630x 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Werner-J. Mayet
    • 1
  • Elisabeth M. Hermann
    • 1
  • Elena Csernok
    • 2
    • 3
  • Wolfgang L. Gross
    • 2
    • 3
  • Karl-H. Meyer zum Büschenfelde
    • 1
  1. 1.I. Medizinische KlinikUniversität MainzMainzGermany
  2. 2.Abteilung für RheumatologieUniversität LübeckBad BramstedtGermany
  3. 3.Med. KrankenhausabteilungRheumaklinik Bad Bramstedt GmbHBad BramstedtGermany

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