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Lactoferrin Co-Purifies with Myeloperoxidase and is Recognised by Anti-Neutrophil Cytoplasm Antibodies

  • Vincent L. M. Esnault
  • Andrew K. Short
  • Sally J. Jones
  • Mark Skehel
  • J. Walker
  • C. Martin Lockwood
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 336)

Summary

Thirty-five sera with binding greater than 20% in a myeloperoxidase (MPO, Calbiochem) ELISA were tested in Western blot analyses. 5/35 blotted MPO, but 5/35 blotted lactoferrin (LF) contaminating the commercial MPO preparation. All the antiLF positive sera, but none of the anti-MPO positive sera, also exhibited anti-nuclear binding on Hep2 cells. Three of the patients with anti-LF antibodies had vasculitis affecting areas additional to the pulmonary-renal involvement which characterised the patients with anti-MPO antibodies.

Keywords

Hep2 Cell Systemic Vasculitis Cyanogen Bromide Positive Seron Western Blot Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Vincent L. M. Esnault
    • 1
  • Andrew K. Short
    • 2
  • Sally J. Jones
    • 2
  • Mark Skehel
    • 3
  • J. Walker
    • 3
  • C. Martin Lockwood
    • 2
  1. 1.Service de Néphrologie-Immunologie CliniqueCHRUNantesFrance
  2. 2.Department of Medicine, Clinical SchoolUniversity of CambridgeUK
  3. 3.MRC laboratory of molecular biologyCambridgeUK

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