Metabolic and Functional Changes in βAPP Overexpressing Mouse Neuronal Cell Line: Muscarinic Receptor-Mediated Calcium Influx
The pathological features of Alzheimer’s disease are the presence of amyloid deposition and neuronal death. Recent reports suggest that several steps of ß amyloid formation from amyloid precursor protein (ßAPP) are involved in the pathophysiology of neuronal death. However, it is now controversial as to which steps are important to induce neuronal death. It is also unknown what kinds of metabolic changes are induced by ßAPP overexpression. To clarify this point we investigated cell metabolism during overexpression of ßAPP using the mouse cholinergic neuronal cell line SN49. Herein, we describe a decrement of muscarinic receptor-mediated calcium influx under the condition of ßAPP overexpression.
KeywordsPertussis Toxin ChAT Activity Muscarinic Receptor Subtype Mouse 6APP695 SN49 Cell
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