PCR for Nematode Identification
Accurate identification of plant pathogenic nematode species is a vital component of many areas of nematology, from systematic research to regulatory nematology and management. It is especially important for management that does not rely on chemical nematicides. In many cases it is desirable to identify to species single females or juveniles. In other cases, it is desirable to identify the presence of small numbers of juveniles in a complex mixture. A major constraint to developing identification techniques for single females and juveniles has been the small size of the nematodes. The availability of the polymerase chain reaction (PCR) amplification technique has revolutionized many aspects of biology by allowing amplification of specific sequences from minute quantities of DNA. This procedure opens new tools for nematology. For example it is now possible to differentiate juveniles of the major root-knot nematode species based on differences in their mitochondrial spacer regions (Powers and Harris, 1993).
KeywordsRAPD Marker Nematode Species Routine Identification Host Race Meloidogyne Species
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