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Structural and Immunochemical Studies on Bovine Lactoferrin Fragments

  • Kei-ichi Shimazaki
  • Makoto Kamio
  • Myong Soo Nam
  • Shinji Harakawa
  • Tetsuya Tanaka
  • Yoshitaka Omata
  • Atsushi Saito
  • Haruto Kumura
  • Katsuhiko Mikawa
  • Ikuo Igarashi
  • Naoyoshi Suzuki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 443)

Abstract

Lactoferrin (Lt) is a metal-binding protein found in milk and other secretory fluids and also in blood. It shows multifunctional properties but the mechanism of developing its function in living systems has not been resolved yet. It is known to exert bacteriostatic effects due to its ability to bind environmental iron. Moreover, apo-lactoferrin has been shown to bind to microbial membranes and causes the direct destruction of microorganisms. Other biological functions attributed to lactoferrin include roles in modulation of the inflammatory response, activation of the immune system, and control of myelopoiesis or cell growth. This molecule is constructed with N- and C-lobes, each of which is composed of 3 domains1. The function of each lobe has been studying and there are certain differences. The biologically significant function has been found mainly in N-lobe. For the aids of resolving their functional analysis, the authors prepared the monoclonal antibodies (mAb) against N-lobe and C-lobe of bovine lactoferrin. To prepare the mAb specific to N-lobe, we used lactoferricin® B (bLfcin, an anti-microbial peptide isolated from N-lobe2) as an antigen3. In this paper, the characterization of the mAb against lactoferrin fragments has been examined and the structure of the mAb-recognition site on lactoferrin molecule was identified.

Keywords

Succinic Anhydride Formic Acid Solution Human Lactoferrin Bovine Lactoferrin Peptide Bond Cleavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Kei-ichi Shimazaki
    • 1
  • Makoto Kamio
    • 1
  • Myong Soo Nam
    • 2
  • Shinji Harakawa
    • 3
  • Tetsuya Tanaka
    • 3
  • Yoshitaka Omata
    • 3
  • Atsushi Saito
    • 3
  • Haruto Kumura
    • 1
  • Katsuhiko Mikawa
    • 1
  • Ikuo Igarashi
    • 4
  • Naoyoshi Suzuki
    • 4
  1. 1.Dairy Science Laboratory Faculty of AgricultureHokkaido UniversitySapporo, 060Japan
  2. 2.Korea Research Institute of Bioscience and BiotechnologyKISTTaejonKorea
  3. 3.Department of Veterinary PhysiologyObihiro University of Agriculture and Veterinary MedicineObihiro, 080Japan
  4. 4.The Research Center for Protozoan Molecular ImmunologyObihiro University of Agriculture and Veterinary MedicineObihiro, 080Japan

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