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The Mechanism of in Vivo Bacteriostasis of Bovine Lactoferrin

  • Tomohiro Ogata
  • Susumu Teraguchi
  • Kouichirou Shin
  • Michiko Kingaku
  • Yasuo Fukuwatari
  • Kouzou Kawase
  • Hirotoshi Hayasawa
  • Mamoru Tomita
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 443)

Abstract

Recently we have reported that orally administered bovine Lf(bLf) exerts bacteriostatic effects against bacterial overgrowth in the intestine of specific-pathogen-free (SPF) mice fed milk. In this animal model, the in vivo bacteriostatic effect of bLf against the proliferation of intestinal Enterobacteriaceae, the bacteria most sensitive to bLf, was independent of the iron-chelating ability of bLf. In addition various proteolytic hydrolysates of bLf (with differing antibacterial activities in vitro) showed the same bacteriostatic effect as undigested bLf. These results suggest that the mechanism of in vivo bacteriostasis of Lf differs from the in vitro mechanism reported.

Keywords

Intestinal Bacterium Bovine Milk Carbohydrate Absorption Cecal Content Bacteriostatic Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Davis, B. D., and E. Z. Ron. 1980. Metabolic regulation, p. 257–287. In B. D. Davis, R. Dulbecco, H. N. Eisen, and H. S. Ginsberg (ed.), Microbiology, third edition. Harper and Row, Publishers, Inc., Philadelphia.Google Scholar
  2. 2.
    Epps, H. M., and E. F. Gale. 1942. The influence of the presence of glucose during growth on the enzyme activities of E. coli: Comparison of the effect with that produced by fermentation acid. Biochem. J., 36: 619–623.PubMedGoogle Scholar
  3. 3.
    France, G. L., D. J. Marmer, and R. W. Steel. 1980. Breast feeding and Salmonella infection. Am. J. Dis. Child. 134: 147–152.PubMedGoogle Scholar
  4. 4.
    Gunn, R. A., A. M. Kimball, R. A. Pollard, J. C. Feeley, and R. A. Feldman. 1979. Bottle feeding as a risk factor for cholera in infants. Lancet. 11: 730–732.CrossRefGoogle Scholar
  5. 5.
    Howie, P. W., J. S. Forsyth, S. A. Ogston, A. Clark, and C. du V. Florey. 1990. Protective effect of breast feeding against infection. Br. Med. J. 300: 11–16.CrossRefGoogle Scholar
  6. 6.
    Magsanik, B. 1961. Catabolite repression. Cold Harbor Symp. Quant. Biol. 26: 249–256.CrossRefGoogle Scholar
  7. 7.
    Masson, P. L., and J. F. Heremans. 1971. Lactoferrin in milk from different species. Comp. Biochem. Physiol. 39: 119–129.CrossRefGoogle Scholar
  8. 8.
    Teraguchi, S., K. Ozawa, S. Yasuda, K. Shin, Y. Fukuwatari, and S. Shimamura. 1994. The bacteriostatic effects of orally administered bovine lactoferrin on intestinal Enterobacteriaceae of SPF mice fed bovine milk. Biosci. Biotech. Biochem. 58: 482–487.CrossRefGoogle Scholar
  9. 9.
    Teraguchi, S., K. Shin, K. Ozawa, S. Nakamura, Y. Fukuwatari, S. Tsuyuki, H. Namihira, and S. Shimamura. 1995. Bacteriostatic effect of orally administered bovine lactoferrin on proliferation of Clostridium species in the gut of mice fed bovine milk. Appl. Environ. Microbiol. 61: 501–506.PubMedGoogle Scholar
  10. 10.
    Teraguchi, S., K. Shin, T. Ogata, M. Kingaku, A. Kaino, H. Miyauchi, Y. Fukuwatari, and S. Shimamura. 1995. Orally administered bovine lactoferrin inhibits bacterial translocation in mice fed bovine milk. Appl. Environ. Microbiol. 61: 4131–4134.PubMedGoogle Scholar
  11. 11.
    Ueda, K., S. Yamazaki, and S. Someya. 1972. Studies on tubercle bacillus infection in germ-free mice. J. Reticuloendothel. Soc. 12: 545–563.PubMedGoogle Scholar
  12. 12.
    Wilson, T. H., and G. Wiseman. 1954. The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surface. J. Physiol. 123: 116–125.PubMedGoogle Scholar
  13. 13.
    Wright, E. M. 1993. The intestinal Na+/glucose cotransporter. Annu. Rev. Physiol. 55: 575–589.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Tomohiro Ogata
    • 1
  • Susumu Teraguchi
    • 1
  • Kouichirou Shin
    • 1
  • Michiko Kingaku
    • 1
  • Yasuo Fukuwatari
    • 1
  • Kouzou Kawase
    • 1
  • Hirotoshi Hayasawa
    • 1
  • Mamoru Tomita
    • 1
  1. 1.Nutritional Science LaboratoryMorinaga Milk Industry Co.Ltd.Zama-City, Kanagawa 228Japan

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