High-Resolution Replication Bands Compared with Morphologic G- and R-bands

  • Régen Drouin
  • Gerald P. Holmquist
  • Claude-Lise Richer
Part of the Advances in Human Genetics book series (AHUG, volume 22)


Replication banding using 5-bromo-2’-deoxyuridine (BrdUrd) has traditionally yielded band patterns with extensive cell-to-cell variation, including variation between homologous chromosomes in the same cell. With the discovery that cycling cells can be blocked at the R/G transition (the time at which R-band synthesis is complete and G-band synthesis has yet to begin), either R-bands or G-bands can be selectively substituted with BrdUrd. This new method (named in this review as the “Thymidine-BrdUrd permutation culture method”) coupled with an improved fluorochrome-photolysis-Giemsa (FPG) staining method that reveals only unsubstituted chromatin, provides proparations with replication band patterns as reproducible and with the same resolution as patterns from standard trypsin-Giemsa banding. In this chapter, we will review the basic cell physiology of how R/G transition blocking and replication banding work and compare replication band patterns with classical band patterns.


Sister Chromatid Giemsa Staining Transmission Electron Microscopy Amniotic Fluid Cell Lateral Asymmetry 
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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Régen Drouin
    • 1
    • 4
  • Gerald P. Holmquist
    • 1
  • Claude-Lise Richer
    • 2
    • 3
  1. 1.Division of BiologyBeckman Research Institute of the City of HopeUSA
  2. 2.Department of AnatomyUniversity of MontrealMontrealCanada
  3. 3.Department of Pathology and Pediatric Research CenterSaint Justine HospitalMontrealCanadaH3T 1C5
  4. 4.Research Unit in Human and Molecular Genetics, Saint Francis of Assisi Hospital Research CenterLaval UniversityQuébec CityCanada

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