Identification and Characterization of an Aeromonas salmonicida Gene Which Affects A-Protein Expression in Escherichia coli
The S-layer of Aeromonas salmonicida (A-layer) is composed of a protein (A-protein) of subunit Mr = 50800. The gene (vapA) coding for A-protein has been cloned, sequenced and expressed in Escherichia coli. Because deletions of flanking DNA 3′ to vapA resulted in decreased A-protein expression in E. coli, we determined the down stream DNA sequence of the 1200 bp of DNA downstream of vap A. This analysis revealed the presence of a gene which codes for a protein with three potential structural domains, i.e., a nucleotide-binding domain, a membrane-associated region, and a leucine zipper-basic region sequence.
KeywordsvapA Gene abcA Gene AEROMONAS SALMONICIDA Tetragonal Surface Represent Amino Acid
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- Blight, M.A., and Holland, I.B., 1990, Structure and function of haemolysin B, Pglycoprotein and other members of a novel family of membrane translocators, Mol. Microbiol. 4: 873.Google Scholar
- Chu, S., Cavaignac, S., Feutrier, J. Phipps, B.M., Kostrzynska, M., Kay, W.W., and Trust, T., 1991, Structure of the tetragonal surface virulence array protein and gene of Aeromonas salmonicida, J. BioL Chem. 266: 15258.Google Scholar