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Identification and Characterization of an Aeromonas salmonicida Gene Which Affects A-Protein Expression in Escherichia coli

  • Shijian Chu
  • Trevor J. Trust
Chapter
Part of the NATO ASI Series book series (NSSA, volume 252)

Abstract

The S-layer of Aeromonas salmonicida (A-layer) is composed of a protein (A-protein) of subunit Mr = 50800. The gene (vapA) coding for A-protein has been cloned, sequenced and expressed in Escherichia coli. Because deletions of flanking DNA 3′ to vapA resulted in decreased A-protein expression in E. coli, we determined the down stream DNA sequence of the 1200 bp of DNA downstream of vap A. This analysis revealed the presence of a gene which codes for a protein with three potential structural domains, i.e., a nucleotide-binding domain, a membrane-associated region, and a leucine zipper-basic region sequence.

Keywords

vapA Gene abcA Gene AEROMONAS SALMONICIDA Tetragonal Surface Represent Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Shijian Chu
    • 1
  • Trevor J. Trust
    • 1
  1. 1.Department of Biochemistry and Microbiology and Canadian Bacterial Disease NetworkUniversity of VictoriaVictoriaCanada

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