Effect of Triton X-100 on the S-Layer of Methanoculleus marisnigri
Methanoculleus marisnigri is an irregularly-shaped coccus originally isolated by Romesser et al. (1979) from the anoxic sediment of the Black Sea. Cells are peritrichously flagellated, and grow optimally at 37° C. The cell wall is composed exclusively of a glycosylated S-layer with hexagonal symmetry. The S-layer protein contains (based on dry weight) 0.8% galactose, 0.3% glucose, and 0.1% mannose (Romesser et al., 1979) and has a Mr = 138 kDa (Zabel et al., 1984). We have isolated the S-layer glycoprotein of this organism by utilizing its sensitivity to Triton X-100 at elevated temperatures.
KeywordsCloud Point Fracture Plane Anoxic Sediment Flagellar Filament Flagellin Protein
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