Advertisement

Scale-up of S-Layer Protein Secretion by Bacillus brevis 47

  • A. J. Daugulis
  • G. K. Whitney
  • A. Wong
  • C. P. Wight
  • B. N. White
Chapter
Part of the NATO ASI Series book series (NSSA, volume 252)

Abstract

Bacillus brevis 47 is a soil bacterium which produces two S-layer wall proteins which are 115 and 104 kDa in size. This strain can, under appropriate cell cultivation conditions, be induced to secrete up to about 14 g/L of these S-layer proteins into the culture medium. This situation has prompted researchers to evaluate this organism as a host for the production and secretion of large quantities of heterologous proteins of commercial value. Proteins which are secreted into the medium are potentially easier to recover and purify than are intracellular proteins. Indeed, the production of recombinant proteins by this organism has been reported (Takao et al., 1989; Yamagata et al., 1989; Konishi et al., 1990), although at substantially lower levels than the native S-layer proteins.

Keywords

Dissolve Oxygen Fructose Concentration Scale Fermentation Fermentor Capacity Bacillus Brevis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Konishi, H., Sato, T., Yamagata, H., and Udaka, S., 1990, Efficient production of human a-amylase by a Bacillus brevis mutant, Appl. Microbial. Biotechnol. 34: 297.CrossRefGoogle Scholar
  2. Miyashiro, S., Enei, H., Hirose, Y., and Udaka, S., 1980, Extracellular production of proteins by microorganisms. Part III. Effect of glycine and L-isoleucine on protein production by Bacillus brevis 47. Agric. Biol. Chem. 44: 105.CrossRefGoogle Scholar
  3. Takao, M., Morioko, T., Yamagata, H., Tsukagoshi, N., and Udaka, S., 1989, Production of swine pepsinogen by protein-producing Bacillus brevis carrying swine pepsinogen cDNA, Appl. Microbiol. Biotechnol. 30: 75.CrossRefGoogle Scholar
  4. Wight, C.P., Daugulis, A.J., Lau, R.H., and White, B.N., 1988, Conditions for production of extracellular protein by Bacillus brevis 47, Biotechnol. Letters 10: 769.CrossRefGoogle Scholar
  5. Wight, C.P., Daugulis, A.J., Lau, R.H., and White, B.N., 1989, Stimulation of extracellular protein production in Bacillus brevis 47, Appl. Microbiol. Biotechnol. 31: 338.CrossRefGoogle Scholar
  6. Wight, C.P., Whitney, G.K., Daugulis, A.J., and White, B.N., 1992, Enhancement and regulation of protein production by Bacillus brevis 47 through manipulation of cell culture conditions, Biotechnol. Bioeng. 40: 46.PubMedCrossRefGoogle Scholar
  7. Yamagata, H., Nakahama, K., Suzuki, Y., Kakinuma, A., Tsukagoshi, N., and Udaka, S., 1989, Use of Bacillus brevis for efficient synthesis and secretion of human epidermal growth factor, Proc. Natl. Acad. Sci. USA 86: 3589.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • A. J. Daugulis
    • 1
  • G. K. Whitney
    • 1
  • A. Wong
    • 1
  • C. P. Wight
    • 1
  • B. N. White
    • 2
  1. 1.Department of Chemical EngineeringQueen’s UniversityKingstonCanada
  2. 2.Department of BiologyMcMaster UniversityHamiltonCanada

Personalised recommendations