Use of Molecular Biology to Study Development and Function of Mammalian Oocytes
Elucidating the genetic program that controls the differentiation of mammalian gametes has been complicated by various factors, including the small size, small numbers, and relative inaccessibility of the cells. In this chapter, I review recent advances in molecular biological approaches and methods that now make this task feasible. Although particular emphasis is placed on analysis at the level of the genes and their mRNAs, analysis of proteins is equally important. Rather than providing a catalogue of the temporal expression of genes during oogenesis, this review illustrates how different approaches have been used to elucidate the pattern of expression of a few of the better-characterized genes.
KeywordsZinc Recombination Germinal Beach Hunt
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