Recommended Deproteinizing Methods for Plasma Guanidino Compound Analysis by Liquid Chromatography

  • T. Hoshino
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 153)


The quantitative determination of guanidino compounds in plasma has been performed in many laboratories working with liquid chromatography and fluorometry ; however, the obtained data were still dispersive for rational discussion. In the course of the determination, several procedures, sample storage, deproteinization, sample charging and so on were thought to cause such variance.


Picric Acid Sulfosalicylic Acid Rational Discussion Sample Charge Guanidino Compound 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    T. Hoshino, M. Sakuma, E. Ohsawa, W. Yamayoshi, K. Sakakibara, T. Sato, H. Sakurai and S. Toyoshima, Effects of hydrogen ion concentration on determination of free guanidino compounds in plasma, Jap. J. Clin. Chem., 9: 198 (1980).Google Scholar
  2. 2.
    A. Ando, U. Mikami, T. Kikuchi, Y. Orita and H. Abe, An automated analytical method for guanidino compound, Jap. J. Clin. Chem., 9: 191 (1980).Google Scholar
  3. 3.
    M. Ariura, Z. Ito, A. Shiotsuki, Y. Takahashi, K. Sakurai and M. Yamagami, The problem of ultrafiltration used as pretreatment in analysis of guanidino compounds, Jap. J. Clin. Chem., 9: 206 (1980).Google Scholar
  4. 4.
    A. Mori and Y. Watanabe, Recovery rates for guanidino compound analyses and variance of measured values by different laboratories, Jap. J. Clin. Chem., 9: 183 (1980).Google Scholar

Copyright information

© Springer Science+Business Media New York 1982

Authors and Affiliations

  • T. Hoshino
    • 1
  1. 1.Pharmaceutical InstituteSchool of Medicine Keio UniversityShinjukuku Tokyo 160Japan

Personalised recommendations