Regulation of the N-Acetylglutamate Content of Rat Hepatocytes by the Glutamate Concentration
It is now generally accepted that the AcGlu concentration plays an important role in the regulation of urea synthesis (1–6). The mechanisms which control the AcGlu content are, however, not completely understood. AcGlu synthetase is specifically activated by arginine (7) but the mitochondrial arginine concentration is much higher than the Ka of arginine for isolated AcGlu synthetase (5). Aoyagi et al. (8) showed that the acetyl-CoA concentration affects the AcGlu content of isolated rat liver cells and Shigesada and coworkers (3) consider glutamate concentration to be an important factor in determining the AcGlu content.
KeywordsUrea Cycle Pyruvate Carboxylase Glutamate Concentration Urea Synthesis Carbamyl Phosphate
Unable to display preview. Download preview PDF.
- 7.M. Tatibana, K. Shigesada, and M. Mori, Acetylglutamate synthetase, in:“The Urea Cycle”, S. Grisola, R. Baguena, and F. Mayor, eds., Wiley, New York (1976).Google Scholar
- 10.H. A. Krebs, N. W. Cornell, P. Lund, and R. Hems, Isolated liver cells as experimental material, in:“Regulation of Hepatic Metabolism, Alfred Benzon Symposium VI”, F. Lundquist, and N. Tygstrup, eds., Academic Press, New York (1974).Google Scholar
- 15.R. M. Archibald, Determination of citrulline and allantoin and the demonstration of citrulline in blood plasma, J. Biol. Chem. 156: 121 (1944).Google Scholar
- 16.H. U. Bergmeyer, “Methoden der enzymatischen Analyse”, 2nd ed., Verlag Chemie, Weinheim (1970).Google Scholar
- 21.G. J. Barritt, G. L. Zander, and M. F. Utter, The regulation of pyruvate carboxylase activity in gluconeogenic tissues, in: “Gluconeogenesis - its regulation in mammalian species”, R. W. Hanson, and M. A. Mehlman, eds., Wiley, New York (1976).Google Scholar