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Structural Relationships between the Chick Oviduct Progesterone Receptor A and B Proteins

  • Wayne V. Vedeckis
  • W. T. Schrader
  • Bert W. O’Malley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 117)

Summary

The chick oviduct contains two distinct forms of the progesterone receptor, termed progestophilins A (Mr = 79,000) and B (Mr = 117,000). Although these two hormone-binding proteins differ significantly in physico-chemical characteristics, a good deal of similarity exists between these two molecules. Thus, both proteins display identical hormone-binding kinetics and steroid specificity. The hormone-binding fragments obtained after the action of an endogenous oviduct Ca2+-activated protease are indistinguishable as analyzed by gel filtration chromatography. Both the A and B proteins are capable of binding to DNA-cellulose, although they elute at very different salt concentrations when subjected to gradient elution. In spite of these similarities all attempts to demonstrate the conversion of progestophilin B to A or to show a common precursor to both have been unsuccessful. Therefore, either the A and B receptor proteins are separate products of closely related genes or the conversion of one form to another occurs very rapidly either in vivo or in vitro.

Keywords

Sodium Dodecyl Sulfate Progesterone Receptor Receptor Protein Ammonium Sulfate Major Protein Band 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Vedeckis, W.V., Schrader, W.T., and O’Malley, B.W. (1978) in Biochemical Actions of Hormones (Litwack, G., ed.) Vol. V, pp. 321–372, Academic Press, N.Y.CrossRefGoogle Scholar
  2. 2.
    Schrader, W.T., Heuer, S.S., and O’Malley, B.W. (1975) Biol. Reprod 12: 134.PubMedCrossRefGoogle Scholar
  3. 3.
    Buller, R.E., Schwartz, R.J., Schrader, W.T. and O’Malley, B.W. (1976) J. Biol. Chem. 251: 5178.PubMedGoogle Scholar
  4. 4.
    Birnbaumer, M.E., Schrader, W.T., and O’Malley, B.W. Submitted for publication.Google Scholar
  5. 5.
    Schrader, W.T. (1975) Methods Enzymol. 36: 187.PubMedCrossRefGoogle Scholar
  6. 6.
    Weber, K. and Osborn, M. (1969) J. Biol. Chem. 244: 4406.PubMedGoogle Scholar
  7. 7.
    Kuhn, R.W., Schrader, W.T., Coty, W.A., Conn, P.M., and O’Malley, B.W. (1977) J. Biol. Chem. 252: 308.PubMedGoogle Scholar
  8. 8.
    Schrader, W.T., Kuhn, P..W., and O’Malley, B.W. (1977) J. Biol. Chem. 252: 299.PubMedGoogle Scholar
  9. 9.
    Coty, W.A., Schrader, W.T., and O’Malley, B.W. (1978) J. Steroid Biochem., in press.Google Scholar
  10. 10.
    Vedeckis, W.V., Schrader, W.T., and O’Malley, B.W. Submitted for publication.Google Scholar
  11. 11.
    Sherman, M.R., Corvol, P.L., and O’Malley, B.W. (1970) J. Biol. Chem. 249: 5351.Google Scholar
  12. 12.
    Schrader, W.T. and O’Malley, B.W. (1972) J. Biol. Chem. 247: 51.Google Scholar
  13. 13.
    Hansen, P.E., Johnson, A., Schrader, W.T., and O’Malley, B.W. (1976) J. Steroid Biochem. 7: 723.PubMedCrossRefGoogle Scholar
  14. 14.
    Schrader, W.T., Toft, D.O., and O’Malley, B.W. (1972) J. Biol. Chem. 247: 2401.Google Scholar
  15. 15.
    Spelsberg, T.C., Webster, R.A., and Pikier, G.M. (1974) in Acidic Proteins of the Nucleus (Cameron, I.L. and Jeter, J.R., Jr., eds.) pp. 247–296, Academic Press, N.Y.Google Scholar
  16. 16.
    Erdos, T., Best-Belpomme, M. and Bessada, R. (1970) Anal. Biochem. 37: 244.PubMedCrossRefGoogle Scholar
  17. 17.
    Sherman, M.R., Atienza, S.B.P., Shansky, J.R., and Hoffman, L. M. (1974) J. Biol. Chem. 249: 5351.Google Scholar
  18. 18.
    Sherman, M.R., Tuazon, F.B., Diaz, S.C., and Miller, L.K. (1976) Biochemistry 15: 980.PubMedCrossRefGoogle Scholar
  19. 19.
    Sherman, M.R. and Diaz, S.C. (1977) Ann. N.Y. Acad. Sci. 286: 81.Google Scholar
  20. 20.
    Vedeckis, W.V., Freeman, M.R., Schrader, W.T., and O’Malley, B.W. In preparation.Google Scholar
  21. 21.
    Hartley, B.S. (1960) Ann Rev. Biochem. 29: 45.PubMedCrossRefGoogle Scholar
  22. 22.
    Sherman, M.R., Pickering, L.A., Rollwagen, F.M., and Miller, L.K. (1978) Fed. Proc. 37: 167.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Wayne V. Vedeckis
    • 1
  • W. T. Schrader
    • 1
  • Bert W. O’Malley
    • 1
  1. 1.Department of Cell BiologyBaylor College of MedicineHoustonUSA

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