Circannual Rhythms in Progesterone Receptor Levels and Functions

  • T. C. Spelsberg
  • P. A. Boyd
  • F. Halberg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 117)


Studies in this laboratory on the nuclear binding sites (acceptors) for progesterone receptor in the developed chick oviduct have resulted in the detection of seasonal variations in the levels and functions of the receptor. Cytosol preparations obtained from the chick oviducts during the winter/spring period between January and May display reduced receptor levels as well as a loss of the capacity of the rec9tor to bind to nuclear “acceptor” sites in vitro. The binding of [3H]P-R to whole chromatin or purified acceptor proteins reannealed to DNA display the same rhythm. No such rhythm is detected for the binding of P-R to pure DNA. Computer analysis of the data, using least squares method to fit the data to cosine curves, shows a significant fit indicating a circannual rhythm in P-R binding to the acceptor protein-DNA complex but not tg pure DNA. The nuclear binding in vivo, achieved by injecting [3H]progesterone into the wing vein and analyzing the radioactivity localized in the oviduct nuclei, also displays a similar rhythm. These results support that native nuclear acceptor sites for progesterone in the chick oviduct represent protein-DNA complexes and not pure DNA. The failure of P-R to bind the nuclear acceptor sites in vivo and in vitro during this period can be explained by the two subunit hypothesis of Schrader and O’Malley, whereby one of the two subunits is absent or inactive during this period.


Nuclear Binding Nonhistone Protein Circannual Rhythm Cosine Curve Receptor Species 


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  1. 1.
    Jensen, E.V. and DeSombre, E.R. (1972) Ann. Rev. Biochem. 41: 203Google Scholar
  2. 2.
    Spelsberg, T.C. and Toft, D.O. (1976) in Receptors and mechanism of action of steroid hormones. J.R. Pasqualini ed., Marcel Dekker, Inc. N.Y. p. 262.Google Scholar
  3. 3.
    Gorski, J. and Gannon, F. (1976) Ann. Rev. Physiol 38: 425.Google Scholar
  4. 4.
    Yamamoto, K.R. and Alberts, B.M. (1976) Ann. Rev. Biochem. 45: 722.Google Scholar
  5. 5.
    O’Malley, B.W. and Means, A.R. (1974) Science 183: 610.PubMedCrossRefGoogle Scholar
  6. 6.
    Williams, D. and Gorski, J. (1974) Biochemistry 13: 5537Google Scholar
  7. 7.
    Stein, G.S., Spelsberg, T.C., and Kleinsmith, L.J. (1974) Science 183: 817.PubMedCrossRefGoogle Scholar
  8. 8.
    Puca, G.A., Sica, V., and Nola, E. (1974) Proc. Nat. Acad. Sci. 71: 979.Google Scholar
  9. 9.
    Puca, G.A., Nola, E., Hibner, U., Cicala, G., and Sica, V. (1975) J. Biol. Chem. 250: 6452.Google Scholar
  10. 10.
    Mainwaring, W.I.P., Symes, E.K., and Higgins, S.J. (1976) Biochem. J. 156: 129.PubMedGoogle Scholar
  11. 11.
    Klyzesejko-Stefanowicz, L., Chui, J.F., Tsai, Y.H., and Hnilica, L.S. (1976) Proc. Nat. Acad. Sci. 73: 1954.Google Scholar
  12. 12.
    Spelsberg, T.C., Webster, R., Pikler, G., Thrall, C., and Wells, D. (1977) Ann. N.Y. Acad. Sci. 286: 43.Google Scholar
  13. 13.
    Szego, C.M. (1976) Gynecol. Invest. 7: 251.PubMedCrossRefGoogle Scholar
  14. 14.
    Gehring, U. and Tomkins, G.M. (1974) Cell 3: 301.PubMedCrossRefGoogle Scholar
  15. 15.
    Yamamoto, K.R., Gehring, U., Stampfer, M.R., and Libley, C.H. (1976) Rec. Prog. Hormone Res. 32: 3.Google Scholar
  16. 16.
    Honma, Y., Kasukabe, T., Okabe, J., and Hogumi, M. (1977) J. Cell Physiol. 9: 227.CrossRefGoogle Scholar
  17. 17.
    Bardin, C.W. and Bullock, L.P., (1974) J. Invest. Dermatol. 63: 75.Google Scholar
  18. 18.
    Hughes, A., Jacobson, H.I., Wagner, R.K., and Jungblut, P.W. (1976) Molec. Cellular Endocrin. 5: 379.CrossRefGoogle Scholar
  19. 19.
    Spelsberg, T.C., Steggles, A.W., and O’Malley, B.W. (1971) J. Biol. Chem. 246: 4188.Google Scholar
  20. 20.
    Spelsberg, T.C., Steggles, A.W., Chytil, F., and O’Malley, B.W., J. Biol. Chem. 247: 1368.Google Scholar
  21. 21.
    Schrader, W.T., and O’Malley, B.W. (1972) J. Biol. Chem. 247: 51.Google Scholar
  22. 22.
    Schrader, W.T., Toft, D.O., and O’Malley, B.W. (1972) J. Biol. Chem. 247: 2401.Google Scholar
  23. 23.
    O’Malley, B.W., Spelsberg, T.C., Schrader, W.T., Chytil, F., and Steggles, A.W. (1972) Nature 253: 141.CrossRefGoogle Scholar
  24. 24.
    Pikier, G.M., Webster, R.A., and Spelsberg, T.C. (1976) Biochem. J. 156: 399.Google Scholar
  25. 25.
    Webster, R.A., Pikler, G.M., and Spelsberg, T.C. (1976) Biochem. J. 156: 409.PubMedGoogle Scholar
  26. 26.
    Spelsberg, T.C., Webster, R.A., and Pikier, G.M. (1976) Nature 262: 65.PubMedCrossRefGoogle Scholar
  27. 27.
    Spelsberg, T.C., Webster, R., Pikier, G., Thrall, C., and Wells, D. (1976) J. Steroid Biochem. 7: 1091.PubMedCrossRefGoogle Scholar
  28. 28.
    Spelsberg, T.C. (1976) Biochem. J. 156: 391.PubMedGoogle Scholar
  29. 29.
    Spelsberg, T.C. and Halberg, F. (in preparation).Google Scholar
  30. 30.
    Boyd, P.A. and Spelsberg, T.C. (in preparation).Google Scholar
  31. 31.
    Boyd, P.A. and Spelsberg, T.C. (1978) 60th Annual Meeting of the Endocrine Society, Miami, Florida, Abstract #519.Google Scholar
  32. 32.
    Spelsberg, T.C. (in preparation).Google Scholar
  33. 33.
    Jackson, V. and Chalkley, R. (1974) J. Biol. Chem. 249: 1615.Google Scholar
  34. 34.
    Jackson, V. and Chalkley, R. (1974) J. Biol. Chem. 249: 1627.Google Scholar
  35. 35.
    Liao, S., Liang, T., and Tymoczko, J.L. (1973) Nature New Biol. 241: 211.PubMedCrossRefGoogle Scholar
  36. 36.
    Monder, J.J. and Hall, R.H. (1973) Can. J. Biochem. 51: 709.CrossRefGoogle Scholar
  37. 37.
    Sluyser, M. (1969) Biochim. Biophys. Acta 182: 235.Google Scholar
  38. 38.
    Mainwaring, W.I.P., Symes, E.K., and Higgins, S.J. (1976). Biochem. J. 156: 129.PubMedGoogle Scholar
  39. 39.
    Puca, G.A. and Bresciani, F. (1968) Nature 218: 967.PubMedCrossRefGoogle Scholar
  40. 40.
    Defer, N., Dastugue, B., and Kruh, J. (1974) Biochimie 56: 559.PubMedCrossRefGoogle Scholar
  41. 41.
    King, R.J.B., Gordon, J., and Steggles, A.W. (1969) Biochem. J. 114: 649.PubMedGoogle Scholar
  42. 42.
    Liang, T. and Liao, S. (1972) Biochim. Biophys. Acta 277: 590.Google Scholar
  43. 43.
    Spelsberg, T.C. (1974) in Acidic Proteins of the Nucleus, I.L. Cameron and J.R. Jeter, ed., Academic Press, N.Y. p. 247.Google Scholar
  44. 44.
    Spelsberg, T.C., Thrall, C.L., Webster, R.A., and Pikier, G.M. (1977) J. Toxicol. Environ. Health 3: 309.Google Scholar
  45. 45.
    Baxter, J.D., Rousseau, G.G., Bensen, M.C., Garcea, R.L., Ito, J., and Tomkins, G.M. (1972) Proc. Nat. Acad. Sci. 69: 1892.Google Scholar
  46. 46.
    Yamamoto, K.R. and Alberts, B.M. (1974) J. Biol. Chem. 249: 7076.Google Scholar
  47. 47.
    Sumida, C., Gelly, C., and Pasqualini, J.R. (1974) C.R. Acad. Sci., ( Paris) Ser. D, 279: 1793.Google Scholar
  48. 48.
    Gwinner, E. (1977) Naturwissenschaften 64: 544.CrossRefGoogle Scholar
  49. 49.
    Davis, D.E. (1967) Physiol. Zool. 40: 391.Google Scholar
  50. 50.
    Follett, B.K. (1978) in Control of ovulation. D. B. Crighton, N.B. Haynes, G.R. Foxcroft and G.E. Lamming, eds., Butterworths, London, p. 267.Google Scholar
  51. 51.
    Pengelley, E.T. (ed) (1974) in Circannual Clocks, Academic Press, N.Y.Google Scholar
  52. 52.
    Farner, D.S. and Lewis, R.A. (1971) in Photophysiology, A.C. Giese, ed., N.Y. Acad. Sci. Vol. 6, p. 325.Google Scholar
  53. 53.
    Farner, D.S. and Follett, B.K. (1966) J. Animal Sci. 25: 90.Google Scholar
  54. 54.
    Winchester, C.F. (1940) in Univ. Missouri Agricultural Exp. Station Res. Bull. 315: 5.Google Scholar
  55. 55.
    Card, L.E. and Nesheim, M.C. (1972) in Poultry Production, Lea and Febiger, Philadelphia, Pa.Google Scholar
  56. 56.
    Assenmacher, I. (1973) in Breeding Biology of Birds, D.S. Farner, ed. Nat. Acad. Sci. Washington, D.C., p. 158.Google Scholar
  57. 57.
    Halberg, F. (1969) Ann. Rev. Physiol. 31, 675.Google Scholar
  58. 58.
    Halberg, F., Johnson, E.A., Nelson, W., Runge, W., and Sothern, R. (1972) Physiol. Teacher 1: 1.Google Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • T. C. Spelsberg
    • 1
  • P. A. Boyd
    • 1
  • F. Halberg
    • 2
  1. 1.Dept. of Molecular MedicineMayo ClinicRochesterUSA
  2. 2.Depts. of Laboratory Medicine, Pathology, Physiology and BiologyUniversity of MinnesotaMinneapolisUSA

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