The Use of Medroxyprogesterone Acetate to Study Progestin Receptors in Immature, Pregnant, and Adult Rabbit Uterus

  • P. D. Feil
  • C. W. Bardin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 117)


Medroxyprogesterone acetate (MPA)3, a potent synthetic progestin, was employed as the 3H-ligand to investigate the status of the rabbit uterine progestin receptor under various hormonal conditions. MPA dissociated slowly from the cytosol and nuclear progestin receptor obtained from ovariectomized, estrogen-primed rabbits. This slow rate of dissociation permitted a partial characterization of the receptors in pregnant and immature, un-primed rabbit uteri. High affinity progestin binding to 6–7 S and 4 S macromolecular species was detected in cytosol and nuclear extracts, respectively, from these animals. In contrast to the 3 peaks of cytosol receptor activity from estrogen-primed animals which were eluted stepwise from DEAE-cellulose columns, predominantly 2 peaks were obtained with cytosols from pregnant and immature, unprimed uteri. Further studies are required to determine to what extent progestin action is modified through interaction of these binding components with themselves and other cellular proteins.


Nuclear Receptor Nuclear Extract Medroxyprogesterone Acetate Estradiol Benzoate Progestin Receptor 
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Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • P. D. Feil
    • 1
    • 2
  • C. W. Bardin
    • 1
    • 2
  1. 1.Departments of Medicine and BiochemistryThe Milton S. Hershey Medical CenterHersheyUSA
  2. 2.The Population CouncilThe Rockefeller UniversityNew YorkUSA

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