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Continuous-Flow Magnetic Cell Sorting Using Soluble Immunomagnetic Label

  • Maciej Zborowski
  • Lee R. Moore
  • Liping Sun
  • Jeffrey J. Chalmers
Chapter

Abstract

Soluble immunomagnetic labels in application to cell sorting offer potential advantages of binding in proportion to cell surface receptor density, and thus high sensitivity. These advantages could be fully realized in magnetic cell sorting systems based on continuous, steady-state cell separation rather than by cell deposition on magnetic surfaces as it is practiced today. Two continuous-flow magnetic cell sorting systems based on the dipole and the quadrupole magnetic fields were designed, built and tested. The cell model system comprised human peripheral lymphocytes, mouse anti-human cytotoxic T cell monoclonal antibody-fluorescein, and rat anti-mouse immunomagnetic colloid. Changes in the fractional concentration of the cytotoxic T cells before and after magnetic fractionation were measured by flow cytometry. The preliminary results indicated sensitivity of the continuous-flow cell separation following magnetic immunocolloid labeling to population distribution of the cell label.

Keywords

Cell Separation Particle Tracking Velocimetry Magnetic Cell Quadrupole Field Magnetic Cell Sorting 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Maciej Zborowski
    • 1
  • Lee R. Moore
    • 1
  • Liping Sun
    • 2
  • Jeffrey J. Chalmers
    • 2
  1. 1.Department of Biomedical Engineering/Wb-3The Cleveland Clinic FoundationClevelandUSA
  2. 2.Department of Chemical EngineeringThe Ohio State UniversityColumbusUSA

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