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Post-Transcriptional Control of the GAP-43 mRNA by the ELAV-Like Protein HuD

  • Nora Perrone-Bizzozero
  • Rebecca Keller
Part of the Endocrine Updates book series (ENDO, volume 16)

Abstract

Post-transcriptional control by mRNA-binding proteins is critical for shaping the temporal and spatial pattern of expression of a large number of developmentally regulated genes. Among these is the gene for GAP-43, a growth-associated protein expressed in neurons primarily during the initial establishment and remodeling of neural connections. Both transcriptional and post-transcriptional mechanisms control GAP-43 gene expression during development. While promoter activity determines the neural-specific expression of the gene, changes in mRNA stability modulate GAP-43 expression in neurons undergoing process outgrowth in response to growth factors and other signaling agents. For example, in PC12 cells induced to differentiate by nerve growth factor (NGF), GAP-43 mRNA levels are regulated primarily through selective changes in the rate of degradation of the mRNA. This process depends on the activation of protein kinase C (PKC) and is mediated by the interaction of highly conserved sequences in the 3’ untranslated region (3 ‘UTR) of the mRNA with neuronal-specific RNA-binding proteins. One of these proteins was recently identified as the ELAV-like protein HuD. SLAV is an RNA-binding protein that is critical for the development of the nervous system in Drosophila and HuD is one of four human homologs of this protein. This chapter discusses the evidence demonstrating a role for HuD in the control of GAP-43 mRNA stability, gene expression and neuronal differentiation, and presents recent findings on the molecular mechanisms underlying these effects.

Keywords

PC12 Cell Neurite Outgrowth mRNA Metabolism ELAV Protein Translation Initiation Factor eIF4G 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Nora Perrone-Bizzozero
    • 1
  • Rebecca Keller
    • 1
  1. 1.School of MedicineUniversity of New MexicoAlbuquerqueUSA

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