Cytochrome P450 RNA—Protein Interactions

  • Matti A. Lang
  • Françoise Raffalli-Mathieu
Part of the Endocrine Updates book series (ENDO, volume 16)


Humans are constantly exposed to xenobiotics present in the environment, the food, or from clinical use. To counteract the toxic accumulation of foreign molecules, living organisms have evolved a defense system comprising a family of detoxifying enzymes (cytochromes P450, CYPs). Numerous xenobiotics induce CYP genes: the induction is an essential adaptive mechanism that allows organisms to adjust their detoxification capacity according to the needs. Only in a few cases are the induction mechanisms understood to some extent. While it is well admitted that both transcriptional and post-transcriptional (mRNA processing and turnover) control is important for cyp genes expression, very little is known about the post-transcriptional mechanisms Of regulation. We have investigated the post-transcriptional regulation of two CYP genes, CYP1A2 and CYP2A5, and identified RNA-binding proteins interacting with their mRNA in an inducer-dependent manner. The strategy used to characterize the RNA protein interaction and to identify the relevant RNA sequences as well as the regulatory factors are described in this chapter.


Electrophoretic Mobility Shift Assay CYP2A5 mRNA Posttranscriptional Regulation Homeostatic Regulation Heterogeneous Nuclear Ribonucleoprotein 


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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Matti A. Lang
    • 1
  • Françoise Raffalli-Mathieu
    • 1
  1. 1.University of UppsalaUppsalaSweden

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