Regulation of Messenger Rna-Binding Proteins by Protein Kinases A and C

  • Richard A. Jungmann
Part of the Endocrine Updates book series (ENDO, volume 16)


The discovery in 1983 that catecholamines via the second messenger cAMP stabilize the mRNA of the lactate dehydrogenase-A (LDH-A) subunit gene indicated for the first time that intracellular mRNA levels are not only controlled at the level of transcription, but that post-transcriptional regulation of mRNA stability is an equally vital mechanism of gene control (1). This finding pointed to a major new mechanism controlling gene expression through binding of effector agents to plasma membrane receptors, a process that elevates intracellular levels of important second messengers such as diacyl glycerol, cAMP, inositol phosphates, ionized calcium and others. These second messengers in turn activate specific protein kinases leading to the phosphorylation and functional modification of putative trans-regulatory proteins that may participate in modulating the mRNA decay mechanism. Two of the major signal transduction pathways that control gene expression involve activation of protein kinase A (PKA) (2) and protein kinase C (PKC) (3). Indeed, evidence has accumulated over recent years showing that the stability of a number of mRNA species is changed by extracellular signals that activate PKA and/or PKC exerting both positive and negative effects on mRNA stability (1, 4–17). This implicates protein kinase target proteins in the process of mRNA stability regulation. Assuming the existence of phosphoproteins as trans-acting mRNA-binding factors, intriguing questions arise: How do these factors modulate mRNA turnover rates and what is their biochemical nature? Do factors interact with mRNA directly or through intermediates, and what is the molecular mechanism of the key interactions? The major goal of this chapter is to discuss the latest insights into the molecular mechanism of mRNA stabilization by two major signal transduction pathways represented by PKA and PKC.


Glioma Cell Phorbol Ester mRNA Stability Homeostatic Regulation mRNA Stability Regulation 
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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Richard A. Jungmann
    • 1
  1. 1.Northwestern University Medical SchoolChicagoUSA

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