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The Technique of Thin-Layer Chromatography

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Practical Liquid Chromatography

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Abstract

The discovery of thin-layer chromatography (TLC) is attributed to Izmailov and Shraiber(1), who, in 1938, separated plant extracts by spotting them onto adsorbent layers produced by coating microscope slides with a slurry of the adsorbent with water and allowing to dry. Separation of the mixture into concentric zones was achieved by applying drops of certain solvents to the center of the spot.

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References

  1. Izmailov, N. A., and Shraiber, H. S., Farmatsiya 3,1 (1938).

    Google Scholar 

  2. Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J. 38, 224 (1944).

    CAS  Google Scholar 

  3. Kirchner, J. G., and Miller, J. M., Ind. Eng. Chem., 44, 318 (1952).

    Article  CAS  Google Scholar 

  4. Kirchner, J. G., and Miller, J. M., J. Agr. Food Chem.} 1, 512 (1953).

    Article  CAS  Google Scholar 

  5. Kirchner, J. G., Miller, J. M., and Keller, G. I., Anal. Chem. 23, 420 (1951).

    Article  CAS  Google Scholar 

  6. Miller, J. M., and Kirchner, J. G., Anal. Chem. 24, 1480 (1952); 20, 2002 (1954).

    Article  CAS  Google Scholar 

  7. Stahl, E., Pharmazie 11, 633 (1956).

    CAS  Google Scholar 

  8. 8. Stahl, E., Chemiker Z. 82, 323, (1958).

    CAS  Google Scholar 

  9. 9. Pelik, N., Bolliger, H. R., and Mangold, H. K., Advan. Chrom. 3, 85 (1966).

    Google Scholar 

  10. Fairbairn, J. W., and Relph, S. J., J. Chromatog. 33, 494 (1968).

    Article  CAS  Google Scholar 

  11. Peifer, J. J., Microchim. Acta 529 (1962).

    Google Scholar 

  12. {paDe} Zeeuw, R. A., Anal. Chem. 40, 2134 (1968).

    Google Scholar 

  13. Korzun, B. P., and Brody, S., J. Pharm. Sci. 53, 454 (1964).

    Article  CAS  Google Scholar 

  14. Brenner, M., and Niederwieser, A., Experientia 17, 237 (1961).

    Article  CAS  Google Scholar 

  15. Halpaap, H., Chem. Ing. Tech., 35, 488 (1963).

    Article  CAS  Google Scholar 

  16. Killer, F. C. A., and Amos, R., J. Inst. Pet., 52, 315 (1966).

    CAS  Google Scholar 

  17. Wieland, T., and Determan, H., Experientia 18, 431 (1962).

    Article  CAS  Google Scholar 

  18. Niederwieser, A., and Brenner, M., Experientia 21, 50 (1965).

    Article  CAS  Google Scholar 

  19. {paDe} Zeeuw, R. A., Anal. Chem. 40, 915 (1968).

    Google Scholar 

  20. Niederwieser, A., and Honegger, C. G., Advan. Chromatog. 2, 123 (l966).

    Google Scholar 

  21. Niederwieser, A., Chromatographia 2, 23, 362 (1969).

    Article  Google Scholar 

  22. Snyder, L. R., and Saunders, D. L., J. Chromatog. 44, 1 (1969).

    Article  CAS  Google Scholar 

  23. Stahl, E., J. Chromatog. 33, 273 (1968).

    Article  CAS  Google Scholar 

  24. Dallas, M. S. J., J. Chromatog. 17, 267 (1965).

    Article  CAS  Google Scholar 

  25. Giddings, J. C., and Keller, R. A., J. Chromatog. 2, 626 (1959).

    Article  CAS  Google Scholar 

  26. Brenner, M., and Niederwieser, A., Experientia 16, 378 (1960).

    Article  CAS  Google Scholar 

  27. Purdy, S. J., and Truter, E. Y., Analyst 87, 802, (1962).

    Google Scholar 

  28. Goldman, J., and Goodall, R. R., J. Chromatog. 32, 24 (1968); 40. 345 (1969).

    Article  CAS  Google Scholar 

  29. Latner, A. L., Molyneux, L., and Dudfield Rose, J., J. Lab. Clin. Med. 40, 345 (1969); 43, 157 (1954)

    Google Scholar 

  30. Dallas, M.S.J., J. Chromatog. 33, 537 (1968).

    Google Scholar 

  31. Shellard, E.J., and Alam, H. Z., J. Chromatog. 33, 347 (1968).

    Article  CAS  Google Scholar 

  32. Amos, R., J. Inst. Pet. 54, 9 (1968).

    CAS  Google Scholar 

  33. Brain, K. R., and Hardman, R., J. Chromatog. 38, 355 (1968).

    Article  CAS  Google Scholar 

  34. Dobies, R. S., J. Chromatog. 40, 110 (1969).

    Article  CAS  Google Scholar 

  35. Heathcote, J. G., and Haworth, C., J. Chromatog. 43, 84 (1969).

    Article  CAS  Google Scholar 

  36. Favretto, L., Pertoldi Marietta, G., and Favretto Gabrielli, L., J. Chromatog. 46, 255 (1970).

    Article  CAS  Google Scholar 

  37. Janchen, D., and Pataki, G., J. Chromatog. 33, 391 (1968).

    Article  CAS  Google Scholar 

  38. Pataki, G., and Niederwieser, A., {jtJ, Chromatog.} 29, 133 (1967).

    Article  CAS  Google Scholar 

  39. Zurcher, H., Pataki, G., Borko, J., and Frei, R. W., J. Chromatog. 43, 457 (1969).

    Article  Google Scholar 

  40. Sawicki, E., and Johnson, H., J. Chromatog. 23, 142 (1966).

    Article  CAS  Google Scholar 

  41. Frodyma, M. H., and Frei, R. W., J. Chem. Ed. 46, 522 (1969).

    Article  CAS  Google Scholar 

  42. Stahl, E., and Jork, H., Zeiss Information 68, 52 (1968).

    Google Scholar 

  43. Schulze, P. E., and Wenzel, M., Angew. Chem. Internat. Edn., 1, 580 (1962).

    Article  Google Scholar 

  44. Wilde, P. F., Thin Layer Chromatography, Unit Trade Press London p. 29 (l964).

    Google Scholar 

  45. Snyder, F., Anal. Biochem. 9, 182 (1964); 11, 510 (1965).

    Article  Google Scholar 

  46. Amos, R., J. Chromatog. 46, 343 (1970).

    Article  Google Scholar 

  47. Honegger, C. G., Helv. Chim. Acta, 45, 1409 (1962).

    Article  CAS  Google Scholar 

  48. Shellard, E. J., and Alam, M. Z., J. Chromatog. 32, 472 (1968).

    Article  CAS  Google Scholar 

  49. Shellard, E. J., and Alam, M. Z., J. Chromatog. 35, 72 (1968).

    Article  CAS  Google Scholar 

  50. Graham, R. T. J., Bark, L. S., and Tinsley, D. A., J. Chromatog. 39, 218} (1968).

    Article  Google Scholar 

  51. Siggia, S., Quantitatiye Organic Analysis, Wiley, New York (1963).

    Google Scholar 

  52. Sawicki, E., Stanley, J. W., Elbert, W. C., and Pfaff, J. D., Anal. Chem. 36, 497 (1964).

    Article  CAS  Google Scholar 

  53. Tumlinson, J. H., Minyard, J. P., Hedin, P. A., and Thompson, A. C., J. Chromatog. 29, 80 (1967).

    Google Scholar 

  54. Cotgreave, T., and Lynes, A., J. Chromatog. 30, 117 (1967).

    Article  CAS  Google Scholar 

  55. Padley, F. B., J. Chromatog. 39, 37 (1969).

    Article  CAS  Google Scholar 

  56. Amos, R., X. Anal. Chem. 236, 350 (1968).

    CAS  Google Scholar 

  57. Stahl, E. (ed.), Dünnschicht-Chromatographie, Springer-Verlag, Berlin-Heidelberg-New York, 2nd ed. (1967).

    Google Scholar 

  58. Kirchner, J. G., Thin-layer chromatography, in Technique of Organic Chemistry, Vol. XII, Perry, E. S., and Weissberger, A., eds., Wiley-Interscience, New York (1967).

    Google Scholar 

  59. Mangold, H. K., in Ref. 57.

    Google Scholar 

  60. Wood, B. A., in Quantitative Paper and Thin Layer Chromatography, Shellard, E. J., ed., Academic Press, London and New York (1968).

    Google Scholar 

  61. Kaiser, R., in Ref. 57.

    Google Scholar 

Further Reading

  • Stahl, E. (ed.), Dünnschicht-Chromatographie, Springer-Verlag, Berlin-Heidelberg-New York, 2nd ed. (1967) (English edition, 1969); Kirchner, J. G., Thin-layer chromatography, in Technique of Organic Chemistry, Vol. XII, Perry, E. S., and Weissberger, A., eds., Wiley-Interscience, New York (1967). (These two books contain major reviews of the techniques and applications of TLC up until 1966.)

    Google Scholar 

  • Shellard, E. J., (ed.), Quantitative Paper and Thin Layer Chromatography, Academic Press, London and New York (1968). (The proceeds of a symposium which reviewed recent developments in various quantitative techniques in paper and thin-layer chromatography.)

    Google Scholar 

  • Snyder, L. R., R F values in thin-layer chromatography on alumina and silica, in Advan. Chromatog. 4, 3 (1967). (Presents a general theory, which is verified using literature data, for the correlation and prediction of RF values in TLC.)

    CAS  Google Scholar 

  • 3rd International Symposium on Reproducibility in Paper and Thin Layer Chromatography, J. Chromatog. 32, 1–409 (1968). (Original papers and reviews are presented which discuss factors affecting R F values and various quantitative methods of analysis.)

    Google Scholar 

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Authors and Affiliations

  1. Esso Petroleum Company, Limited Esso Research Centre Abingdon, Berkshire, England

    S. G. Perry, R. Amos & P. I. Brewer

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  1. S. G. Perry
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  2. R. Amos
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  3. P. I. Brewer
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Perry, S.G., Amos, R., Brewer, P.I. (1973). The Technique of Thin-Layer Chromatography. In: Practical Liquid Chromatography. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6226-6_6

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  • DOI: https://doi.org/10.1007/978-1-4757-6226-6_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-6228-0

  • Online ISBN: 978-1-4757-6226-6

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