Abstract
Many outsiders with a scientific training looking in at the practice of chromatography must regard the exercise as an art or mystique, rather than as a science. There is no doubt that empiricism and experience frequently provide the dominating guidelines in setting up an experiment; theoretical considerations, even of a rudimentary nature, only infrequently playa major part in the design and operation of a chromatographic separation. This situation is even more pronounced in liquid chromatography than in the newer, but enormously more successfully applied technique of gas chromatography. Indeed, despite its seventy or eighty years of history, liquid chromatography has made very little progress, the materials and equipment in use today (at least for most adsorption work in columns) differing rather little from that of Day and Tswett.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Giddings, J. C., Dynamics of Chromatography. Part I, Marcel Dekker, New York, 1965.
Wilson, J. N., J. Am. Chem. Soc. 62, 1583 (1940).
Martin, A. J. P., and Synge, R. M., Biochem. J. 35, 1358 (1941).
Van Deemter J. J. Zuiderweg F. J. and Klinkenberg A. Chem. Eng. Sci. 5 271 1956
Ref. 1, pp. 242-243.
Wilke C. R. and Chang P. Am. Inst. Chem. Eng. J. 1 264 1955
Ref. I, p. 35.
Frenkel, J., Kinetic Theory of Liquids, Oxford Univ. Press Chapter 4, (1946).
Kelley, R. N., and Billmeyer, F. W., Jr., Anal. Chem. 41, 874 (1969).
Stewart, H. N. M., Amos, R., and Perry, S. G., J. Chromatog. 38, 209 (1968).
Maggs, R. J., and Young, T. E., Gas Chromatography 1968, Harbourn, C. L. A., ed., Inst. Petroleum, London (1969).
Further Reading
Knox, J. H., and Saleem, H., J. Chromatog. Sci. 7, 614 (1969). (Discusses kinetic conditions for optimum speed and resolution in column chromatography.)
Smuts, T. W., van Niekerk, F. A., and Pretorius, V., J. Gas Chroma tog. 5, 190 (1967}). (Discusses the effect of operating parameters on speed of liquid chromatograph
Huber, J. F. K., in Physical Separation Methods, Vol. IIB, Wilson, C. L., and Wilson, D. W., eds., Elsevier, Amsterdam Chapter 1, (1968). (Contains a presentation of the theory of liquid chromatography in columns.)
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1973 Springer Science+Business Media New York
About this chapter
Cite this chapter
Perry, S.G., Amos, R., Brewer, P.I. (1973). Band Spreading in Chromatography. In: Practical Liquid Chromatography. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6226-6_2
Download citation
DOI: https://doi.org/10.1007/978-1-4757-6226-6_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-6228-0
Online ISBN: 978-1-4757-6226-6
eBook Packages: Springer Book Archive