Analysis of Equilibrium Adsorption Isotherms for Human Protein C Purification by Immobilized Metal Affinity Chromatography
Protein C (PC) is a glycoprotein that plays a pivotal role as an anticoagulant, antithromobotic, and thrombolytic therapeutic in the blood coagulation cascade (Esmon, 1989). It is a member of the vitamin K-dependent (VKD) family also consisting of coagulation protein factors VII, IX, X, proteins S, Z and prothrombin. Human PC is synthesized in the liver as a single chain precursor and circulates in the blood primarily as a two chain zymogen that is activated by proteolytic clevage. Activated PC is a potent serine protease that regulates blood coagulation by deactivating factors Va and VIIIa (a: activated form), thus preventing generation of the enzyme factors Xa and thrombin (Wu and Bruley, 1999).
KeywordsDeep Vein Thrombosis Immobilize Metal Affinity Chromatography Equilibrium Adsorption Isotherm Blood Coagulation Cascade Maryland Baltimore County
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