Abstract
Shrimp viral diseases have seriously impacted the sustainability and economic success of the shrimp aquaculture industry worldwide. Among several of the recent viral pathogens which caused massive mortalities in cultured shrimp were the yellow-head virus (YHV) and the white spot baculovirus (WSBV) (also called the Chinese baculovirus [CBV] or the systemic ectodermal and mesodermal baculovirus [SEMBV]). Both YHV and CBV have been reported by our laboratory to be highly pathogenic for Penaeus stylirostris (blue shrimp) and P. vannamei (white shrimp), the two principal penaeid species commercially cultured in Hawaii and the Western Hemisphere (Lu et al. 1997). They are thus potentially a serious problem particularly to the brood stock industry.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chang, P.S., Y.C. Wang, C.F. Lo and G.H. Kou. (1996). Infection of white spot syndrome associated non-occluded baculovirus in cultured and wild shrimps. Second International Conference on the Culture of Penaeid Prawns and Shrimps, SEAFDEC, Iloilo City, Philippines, May 14–17, 1996.
Durand, S., D.V. Lightner, L.M. Nunan, R.M. Redman, J. Mari and J.R. Bonami,. (1996). Dis. Aquatic Org. In press. Laemmli, U.K. (1970). Nature 227: 680–685.
Lo, C.F., J-H. Leu, C-H. Ho, C-H. Chen, S-E. Peng, Y-T. Chen, C-M. Chou, P-Y. Yeh, C-J. Huang, H-Y. Chou, CH. Wang and G-H. Kou. (1996). Dis. Aquatic Org. 25: 133–141.
Lu, Y., L.M. Tapay, P.C. Loh, R.B. Gose and J.A. Brock. 1997. The pathogenicity of a baculo-like virus isolated from diseased penaeid shrimp obtained from China for cultured penaeid species in Hawaii. Aquaculture Internats. 5: 277–282.
Mari, J., R.J. Bonami, and D.V. Lightner. (1993). Virmet 74: 2637–2643.
Mari, J., D.V. Lightner, B.T. Poulos and J.R. Bonami. (1995). Dis. Aquatic Org. 22: 129–134.
Nadala, E.C.B., Jr., Y. Lu, P.C. Loh and J.A. Brock. (1992). Fish Pathol. 27: 143–147.
Poulos, B.T., J. Mari, J.A. Bonami, R.M. Redman and D.V. Lightner. (1994). J. Virol. Meth. 49: 187–194.
Tapay, L.M., Y. Lu, E.C.B. Nadala Jr., J.A. Brock, and P.C. Loh. (1996). Development of an in vitro quantal assay in primary cell cultures for a baculo-like virus of penaeid shrimps. Proc. Second International Conference on the Culture of Penaeid Prawns and Shrimps. Iloilo City, Philippines. p. 53.
Tapay, L.M., E.C.B. Nadala, Jr., S. Cao, and P.C. Loh. (1997). The specific detection of shrimp viral pathogens by Western blot. Oceanology Internatl. 97-Pacific Rim 2: 35–44.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Loh, P.C., Tapay, L.M., Cesar, E., Nadala, B., Lu, Y. (1998). A SDS/Page/Western Blot/EIA Protocol for the Specific Detection of Shrimp Viral Pathogens. In: Gal, Y.L., Halvorson, H.O. (eds) New Developments in Marine Biotechnology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5983-9_63
Download citation
DOI: https://doi.org/10.1007/978-1-4757-5983-9_63
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-3300-3
Online ISBN: 978-1-4757-5983-9
eBook Packages: Springer Book Archive