Advertisement

Biocompatible Alginates for Use in Biohybrid Organs

  • Gerd Klöck
  • Patrik Gröhn
  • Christan Hasse
  • Ulrich Zimmermann
Chapter

Abstract

Using artificial, semi-permeable membranes, transplanted tissue can be physically isolated from the immune system of the recipient (immunoisolation). This allows, in principle, transplantation of cells of different genera, without the need for pharmacological immunosuppression (Clayton et al., 1993; Colton, 1995; Lanza & Chick, 1997). In the past, a whole range of different concepts have been employed to achieve this goal (see Clayton et al., 1993; Colton, 1995; Geisen et al., 1990; Lanza & Chick, 1997). It is now generally accepted that micocapsules composed of natuaral polysacccharides, especially of alginates, are the material of choice (Clayton et al., 1993; Geisen et al., 1990; Lanza & Chick, 1997; Smidsrod & Skjak-Braerk, 1990).

Keywords

Parathyroid Tissue Hydrogel Bead Guluronic Acid Murine Splenocytes Free Flow Electrophoresis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Clayton, H.A., James, R.F., London, N.J., 1993, Islet microencapsulation: a review. Acta Diabetol 30: 181–189PubMedCrossRefGoogle Scholar
  2. Colton, C.K., 1995, Implantable biohybrid artificial organs. Cell Transplantation 4: 415–436PubMedCrossRefGoogle Scholar
  3. De Vos, P, Wolters, G.H., Fritschy, W.M., Van Schilfgaarde, R., 1993, Obstacles in the application of microencapsulation in islet transplantation. 1ntJArtifOrgans 16: 205–212PubMedGoogle Scholar
  4. Geisen, K, Deutschländer, H., Gorbach, S., Klenke, C., Zimmermann, U., 1990, Function of barium alginate microencapsulated xenogenic islets in different diabetic mouse models.. Frontiers in Diabetes Research Lessons from animal diabetes 111: 142–148 (Editor: Shafir, E.)Google Scholar
  5. Hasse, C., Klöck, G., Bartsch, D., Zielke, A., Zimmermann, U., Rothmund, M.,1997, First successful xenotransplantation of microencapsulated human parathyroid tissue in experimental hypoparathyroidism: Long term in vivo function without immunosuppression. J. Microencapsulation (in press).Google Scholar
  6. Hasse, C., Klöck, G., Zielke, A., Schlosser, A., Barth, P., Zimmermann, U., and Rothmund, M., 1996, Transplantation of parathyroid tissue in experimental hypoparathyroidism: in vitro and in vivo function of parathyroid tissue microencapsulated with a novel alginate. Int. J. Artif. Organs 19: 1–7.Google Scholar
  7. Klöck, G., Frank, H., Houben, R., Zekorn, T., Horcher, A., Siebers, U., Wöhrle, M., Federlin, K., Zimmermann, U., 1994, Production of purified alginates suitable for use in immunoisolated transplantation. Applied Microbial. Biotechnol. 40: 639–643.Google Scholar
  8. Klöck, G., Pfeffermann, A., Ryser, C., Gröhn, P., Kuttler, B., Hahn, H.J., and Zimmermann, U. Biocompatibility of mannuronic-acid rich alginates. 1997, Biomaterials 18: 707–713.PubMedCrossRefGoogle Scholar
  9. Lanza, R.P., Chick, W.L. 1997, Transplantation of encapsulated cells and tissues. Surgery 21: 1–9.CrossRefGoogle Scholar
  10. Lim, F, Sun, A.M., 1980, Microencapsulated islets as a bioartificial pancreas. Science 210: 908–910PubMedCrossRefGoogle Scholar
  11. Pfeffermann, A., Klöck, G., Gröhn, P., Kuttler, B., Hahn, H.-J., and Zimmermann. U., 1996, Assay procedures for the assessment of the biocompatibility of alginate implants. Cell Engineering 4: 167–173Google Scholar
  12. Smidsrod, O., Skjak-Braerk, G., 1990, Alginate as immobilization matrix for cells. Trends Biotechnol 8: 71–78PubMedCrossRefGoogle Scholar
  13. Zekorn, T., Horcher, A., Siebers, U., Schnettler, R., Klöck, G., Hering, B., Zimmermann, U., Bretzel, R.G., and Federlin, K. 1992, Barium-cross-linked alginate beads: a simple, one-step method for successful immunoisolated transplantation of islets of Langerhans. Acta Diabetologia 29: 99–106.CrossRefGoogle Scholar
  14. Zimmermann, U., Klöck, G., Federlin, K., Hannig, K., Kowalski, M., Brezel, R.G., Horcher, A., Entenmann, H., Siebers, U. and Zekorn, T.,1992, Production of mitogen contamination free alginates with variable rations of mannuronic to guluronic acid by free flow electrophoresis. Electrophoresis 13: 269–274.Google Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Gerd Klöck
    • 1
  • Patrik Gröhn
    • 1
  • Christan Hasse
    • 2
  • Ulrich Zimmermann
    • 1
  1. 1.Department of BiotechnologyUniversity of WürzburgWürzburgGermany
  2. 2.Department of SurgeryUniversity of MarburgMarburgGermany

Personalised recommendations