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Dereplication and Profiling of Marine Bacteria by Fatty Acid Analysis of Crude Extracts Using Fourier Transform Mass Spectrometry

  • David J. Bourne
  • Eliane Abou-Mansour
  • Russell T. Hill
  • Peter T. Murphy
Chapter

Abstract

Methods for grouping of bacteria include analysis of fatty acids by pyrolysis mass spectrometry of free fatty acids (Sanglier et al., 1992), their methyl esters (Basile et al., 1995), phospholipids and glycolipids by fast atom bombardment mass spectrometry (Heller et al., 1987), measurement of fatty acid content by methylation and gas chromatographic analysis of methyl esters (Bertone et al., 1996), GCMS (Dzierzewicz et al., 1996), and mass spectral fingerprints generated by matrix assisted laser desorption of intact bacterial cells (Claydon et al., 1995). Methods developed for grouping terrestrial bacteria can be transferred to marine species (Basile et al., 1995). There has been considerable recent interest in methods for phylogenetic grouping and identification of marine bacteria, where traditional biochemical tests are not effective in grouping isolates.

Keywords

Marine Bacterium Total Fatty Acid Content High Mass Accuracy FOURIER Transform Mass Spectrometry Fast Atom Bombardment Mass Spectrometry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Basile F., Voorhees K. J. and Hadfield T. L. 1995, Appl. Environ. Microbiol. 61: 1534–1539PubMedGoogle Scholar
  2. Bertone S., Giacomini M., Ruggiero C., Piccarolo C. and Calegari L. 1996, Appl. Environ. Microbiol. 62: 2122–2132Google Scholar
  3. Claydon M. A., Davey S. N., Edward-Jones V. and Gordon D. B. 1996, Nature Biotech. 14 (11) 1584–1586CrossRefGoogle Scholar
  4. Dzierzewicz Z., Cwalina B., Kurkiewicz S., Chodurek E. and Wilczok T. 1996, App!. Environ. Microbiol. 62: 3360–3365Google Scholar
  5. Heller D. N., Cotter R. J., Fenselau C., Platt J. A, and Uy O. M. 1987, Anal. Chem. 60: 1415–1419Google Scholar
  6. Sanglier Jean-J., Whitehead D., Saddler G. S., Ferguson E. V. and Goodfellow M. 1992, Gene 115: 235–242Google Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • David J. Bourne
    • 1
  • Eliane Abou-Mansour
    • 1
  • Russell T. Hill
    • 1
  • Peter T. Murphy
    • 1
  1. 1.Australian Institute of Marine ScienceTownsvilleAustralia

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