Abstract
Imaging neurotransmitter receptors has progressed steadily over the past 15 or so years. The earliest studies involved autoradiographic localization following in vivo administration of radiolabelled highly specific, high affinity ligands for specific receptors (Figure 1). Their approach, however, is invasive and therefore applicable to tissues obtained at surgery or at autopsy. Subsequently developed in vitro labelling autoradiographic techniques were more powerful and versatile but still had the limitation of being invasive (1). Thus Positron Emission Tomography (PET) has a remarkable capacity to carry receptor studies to the clinical level (Figure 2). This paper describes some of the advantages of PET and the progress made in several areas. It also shows how PET can be a powerful extension of biochemical, in vitro receptor binding studies in homogenates.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
M. J. Kuhar, E. B. DeSouza, J. R. Unnerrtall, Neurotransmitter Receptor Mapping by Autoradiography and Other Methods, Ann. Rev. Neurosci. 9: 27 (1986).
D. F. Wong, A. Gjedde, H. N. Wagner, Jr, Quantification of Neuroreceptors in the Living Human Brain. I. Irreversible Binding of Ligands, J. Cereb. Blood Flow and Metab. 6: 137 (1986).
D. F. Wong, A. Gjedde, H. N. Wagner, Jr., R. F. Dannals, K. H. Douglass, J. M. Links, and M. J. Kuhar, Quantification of Neuroreceptors in the Living Human Brain. II. Inhibition Studies of Receptor Density and Affinity, J. Cereb. Blood Flow and Metab. 6: 147 (1986).
J. C. Crawley, T. Smith, N. Veall, G. D. Zanell, T. J. Crow, and F. Owen, Dopamine Receptors Displayed in Living Human Brain With 77Br-PBromospiperone, Lancet. 2: 975 (1983).
J. C. W. Crawley, T. J. Crow, E. Johnstone, S. R. D. Oldland, F. Owen, D. G. C. Owens, M. Poulter, T. Smith, N. Veall, and G. D. Zanell, Dopamine Receptors in Schizophrenia Studies in Vivo. Lancet. 2: 224 (1986).
J. C. Mazziotta, M. E. Phelps, D. Plummer, D. E. Kuhl, Quantification in Positron Computed Tomography. 3. Physical-Anatomical Effects, J. of Computer Assisted Tomography. 5: 734 (1981).
S. Z. Lever, H. D. Burns, T. M. Kervitsky, H. W. Goldfarb, D. V. Woo, D. F. Wong, L. A. Epps, A. V. Kramer, H. N. Wagner, Jr., Design, Preparation, and Biodistribution of a Technetium-99m Triaminedithiol Complex to Assess Regional Cerebral Blood Flow, J. Nucl. Med. 26: 1287 (1985).
H. N. Wagner Jr., H. D. Burns, R. F. Dannals, D. F. Wong, B. Langstrom, T. Duelfer, J. J. Frost, H. T. Ravert, J. M. Links, S. B. Rosenbloom, S. E. Lukas, A. V. Kramer, and M. J. Kuhar, Imaging Dopamine Receptors in the Human Brain by Positron Tomography, Science. 221: 1264 (1983).
M. A. Mintun, M. E. Raichle, M. R. Kilbourn, G. F. Wooten, M. J. Welch, A Quantitative Model for the In Vivo Assessment of Drug Binding Sites with Positron Emission Tomography, Ann. Neurol. 15: 217 (1984).
L. Farde, H. Hall, E. Ehrin, G. Sedvall, Quantitative Analyses of D2 Dopamine Receptor Binding in the Living Human Brain by PET, Science. 231: 258 (1986).
D. F. Wong, H. N. Wagner Jr., R. F. Dannals, J. M. Links, J. J. Frost, H. T. Ravert, A. A. Wilson, A. E. Rosenbaum, A. Gjedde, K. H. Douglass, J. D. Petronis, M. F. Folstein, J. K. Thomas Toung, H. Donald Burns and M. J. Kuhar, Effects of Age on Dopamine and Serotonin Receptors Measured by Positron Tomography in the Living Human Brain, Science. 226: 1393 (1984).
D. F. Wong, H. N. Wagner Jr., R. F. Dannals, J. M. Links, J. J. Frost, H. T. Ravert, A. A. Wilson, A. E. Rosenbaum, A. Gjedde, K. H. Douglass, J. D. Petronis, M. F. Folstein, J. K. Thomas Toung, H. Donald Burns and M. J. Kuhar, Effects of Age on Dopamine and Serotonin Receptors Measured by Positron Tomography in the Living Human Brain, Science. 226: 1393 (1984).
D. F. Wong, E. P. Broussolle, G. Wand, V. Villemagne, R. F. Dannals, J. M. Links, H. A. Zacur, J. Harris, S. Naidu, C. Braestrup, H. N. Wagner Jr., and Albert Gjedde, In Vivo Measurement of Dopamine Receptors in Human Brain by Positron Emission Tomography Age and Sex Differences, In: “Central Determinants of Age-Related Declines in Motor Function,” Volume 515, JA Joseph, Ed., Ann NY Acad Sci, NY (1988).
D. F. Wong, G. Pearlson, L. E. Tune, V. Villemagne, C. Ross, R. F. Dannals, J. Links, H. Ravert, A. Wilson, H. N. Wagner, Jr., A. Gjedde, In Vivo Measurement of D2 Dopamine Receptor Abnormalities in Drug Naive and Treated Manic-Depressive Patients, J. Nucl. Med. 28:611 (1987).
A. P. Wolf, C. Y. Shiue, J. S. Fowler, J. A. G. Russell, R. R. MacGregor, C. D. Arnett, and J. Logan, The Relationship Between Dopamine D-2 Receptor Occupancy by Antipsychotic Drugs and Plasma Drug Levels as Determined by 18F-NMethyl-Spiroperidol (18F-NMS) and Positron Emission Tomography (PET), J. Cereb. Blood Flow and Metab. 7: 361 (1987).
K. L. Leenders, W. H. Poewe, A. J. Palmer, D. P. Brenton, R. S. Frackowiak, Inhibition of L-[18F] Fluorodopa Uptake into Human Brain by Amino Acids Demonstrated by Positron Emission Tomography, Ann. Neurol. 20 (2): 258 (1986).
D. E. Kuhl, M. E. Phelps, C. H. Markham, E. J. Metter, W. H. Riege, J. Winter, Cerebral Metabolism and Atrophy in Huntington’s Disease Determined by 18FDG and Computed Tomographic Scan, Ann. Neuro. 12: 425 (1982).
M. R. Hayden, J. Hewitt, A. J. Stossl, C. Clark, W. Amnann, W. R. M. Martin, The Combined Use of Positron Emission Tomography and DNA Polymophisins for Predimical Detection of Huntington’s Disease, Neurology. 37: 1441 (1987).
J. C. Mazziotta, M. E. Phelps, J. J. Pahl, Sung- Cheung Huang, L. R. Baxter, W. H. Riege, J. M. Hoffman, D. E. Kuhl, A. B. Lanto, J. A. Wapenski, and C. H. Markham, Reduced Cerebral Glucose Metabolism in Asymtomatic Subjects at Risk for Huntington’s Disease, The New England J. Med. 316: 357 (1987).
A. B. Young, J. B. Penney, S. Starosta-Rubinstein, D. Markel, S. Berent, J. Rothley, A. Betley, R. Hichwa, Normal Caudate Glucose Metabolism in Persons at Risk for Huntington’s Disease, Arch. Neurol. 44: 254 (1987).
D. F. Wong, J. M. Links, H. N. Wagner Jr., S. E. Folstein, S. Suneja, R. F. Dannals, H. T. Ravert, A. A. Wilson, L. E. Tune, G. Pearlson, M. F. Folstein, A. Bice, M. J. Kuhar, Dopamine and Serotonin Receptors Measured in Vivo in Huntington’s Disease with C-11 N-Methylspiperone PET Imaging, J. Nucl. Med. 26: P107, (1985).
D. F. Wong, G. Pearlson, L. E. Tune, In Vivo Measurement of D2 Dopamine Receptor Abnormalities in Drug Naive and Treated Manic-Depressive Patients, J. Nucl. Med. 28:P611, (1987).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer Science+Business Media New York
About this chapter
Cite this chapter
Wong, D.F., Kuhar, M.J. (1988). In Vivo PET and SPECT Receptor Imaging: New Technology and Tactics for Receptor Measurement. In: Kito, S., Segawa, T., Kuriyama, K., Tohyama, M., Olsen, R.W. (eds) Neuroreceptors and Signal Transduction. Advances in Experimental Medicine and Biology, vol 236. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5971-6_15
Download citation
DOI: https://doi.org/10.1007/978-1-4757-5971-6_15
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-5973-0
Online ISBN: 978-1-4757-5971-6
eBook Packages: Springer Book Archive