Development of NILE Glycoprotein in Chick Brain
The nerve growth factor (NGF)-inducible large external (NILE) glycoprotein is a cell surface component and it was first identified in the NGF-induced neuronal differentiation of the PC12 pheochromocytoma cell line1,2. It has been shown to be widely distributed in the mammalian central and peripheral nervous system, but not in nonneural tissues3. Recent reports have indicated a role for this molecule in fasciculation of neurites in culture, and its appearance in the developing mammalian central nervous system appears to be correlated with genesis of fiber tracts4,5,6,7. Furthermore reports from several groups7’8,9,10 have indicated that this molecule is immunologically indistiguishable from the high-molecular weight component of the brain L1 antigen and the neuron-glia cell adhesion molecule (Ng-CAM). Both of these antigens are also believed to have a role in regulating cell-cell adhesion and fasciculation in the developing nervous system10,11,12. With these considerations in mind we became interested in the understanding of the mechanisms which regulate NILE development in the central nervous system. A first step towards the understanding of this kind of regulatory mechanism, is the study of the spatiotemporal distribution of the protein under investigation in a specific tissue. For such developmental studies chick brain appears to provide certain advantages. Chick brain develops mostly during the in ovo period of life and is therefore a closed system, which offers also the opportunity for making experimental manipulations during embryogenesis. Furthermore certain areas of chick brain have been well characterized developmentally and anatomically. The results which we report here describe the spatiotemporal distribution of NILE in chick cerebellum and optic lobes.
KeywordsNerve Growth Factor Optic Lobe Optic Tectum External Granular Layer Chick Brain
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