Lipid Composition of Membrane Domains
The isolation of subfractions of cell membranes on the basis of their solubility in non-ionic detergents has led to the discovery of lipid domain structure in membranes. Detergents used for this purpose include Triton, Brij, Lubrol and CHAPS. Different lipid constituents are known to resist solubilization by different detergents and the resulting fractions may associate with different membrane proteins. In general, the detergent-resistant membrane fractions tend to be dominated by saturated molecular species of sphingomyelin and phosphatidylcholine and invariably include significant proportions of cholesterol. The lipid composition is consistent with formation of liquid-ordered phases. The present evidence favours a model in which the lateral segregation of membrane proteins takes place on the basis of their affinity for liquid-ordered lipid domains within the membrane.
KeywordsLipid Composition Lipid Raft Membrane Domain Membrane Raft Lipid Domain
Unable to display preview. Download preview PDF.
- Ahmed, S. N., Brown, D. A. and London, E., 1997, On the origin of sphingolipid/cholesterolrich detergent-insoluble cell membranes: physiological concentrations of cholesterol and sphingolipid induce formation of a detergent-insoluble, liquid-ordered lipid phase in model membranes, Biochemistry, 36: 10944–10953.PubMedCrossRefGoogle Scholar
- Braccia, A., Villani, M, Immerdal, L., Niels-Christiansen, L. L., Nystrom, B. T., Hansen, G. H. and Danielsen, E. M., 2003, Microvillar membrane microdomains exist at physiological temperature. Role of galectin-4 as lipid raft stabilizer revealed by “superrafts”, J Biol Chem, 278: 15679–15684.PubMedCrossRefGoogle Scholar
- Rouquette-Jazdanian, A. K., Pelassy, C., Breittmayer, J. P., Cousin, J. L. and Aussel, C., 2002, Metabolic labelling of membrane microdomains/rafts in Jurkat cells indicates the presence of glycerophospholipids implicated in signal transduction by the CD3 T-cell receptor, Biochem J, 363: 645–655.PubMedCrossRefGoogle Scholar
- Schroeder, R. J., Ahmed, S. N., Zhu, Y., London, E. and Brown, D. A., 1998, Cholesterol and sphingolipid enhance the Triton X-100 insolubility of glycosylphosphatidylinositolanchored proteins by promoting the formation of detergent-insoluble ordered membrane domains, JBiol Chem, 273: 1150–1157.CrossRefGoogle Scholar
- Xu, X., Bittman, R., Duportail, G., Heissler, D., Vilcheze, C. and London, E., 2001, Effect of the structure of natural sterols and sphingolipids on the formation of ordered sphingolipid/sterol domains (rafts). Comparison of cholesterol to plant, fungal, and disease-associated sterols and comparison of sphingomyelin, cerebrosides, and ceramide, JBiol Chem, 276: 33540–33546.CrossRefGoogle Scholar
- Zubiaur, M., Fernandez, O., Ferrero, E., Salmeron, J., Malissen, B., Malavasi, F. and Sancho, J., 2002, CD38 is associated with lipid rafts and upon receptor stimulation leads to Akt/ protein kinase B and Erk activation in the absence of the CD3-zeta immune receptor tyrosine-based activation motifs, JBiol Chem, 277: 13–22.CrossRefGoogle Scholar