Voltage-Sensitive Ion Channels in Human B Lymphocytes

  • Jeffrey B. Sutro
  • Bharathi S. Vayuvegula
  • Sudhir Gupta
  • Michael D. Cahalan


Ion channels are protein pores that provide for the rapid movement of ions across cell membranes. Such channels control the transmembrane flow of ions by opening or closing in response to appropriate stimuli (a property referred to as gating) and by allowing only specific types of ions to pass through (a property referred to as selectivity). Although ion channels have been studied in nerve and muscle for many years (see 1 for a review), it is only since the development of the patch-clamp technique (2) that they have been detected in a variety of other tissues and systems. In the immune system such diverse cell types as T lymphocytes, B lymphocytes, macrophages, neutrophils, hybridomas, and natural killer cells express an assortment of channels (reviewed in 3).


Outward Current External Solution Tail Current Single Channel Conductance Channel Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Hille, B: Ionic channels of excitable membranes. Sinauer Associates, Sunderland Mass. (1984).Google Scholar
  2. 2.
    Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ: Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pfluegers Arch. 391: 85 (1981).CrossRefGoogle Scholar
  3. 3.
    Cahalan MD, Chandy KG, DeCoursey TE, Gupta S, Lewis R, Sutro JB: Ion channels in T lymphocytes. In: Gupta S, Paul WE, and Fauci AS (Eds.), Mechanisms of Lymphocyte Activation and Immune Regulation. New York: Plenum Press, 1987.Google Scholar
  4. 4.
    DeCoursey TE, Chandy KG, Gupta S, Cahalan MD: Voltage-gated K+ channels in human T lymphocytes: a role in mitogenesis? Nature 307: 465 (1984).PubMedCrossRefGoogle Scholar
  5. 5.
    Chandy KG, DeCoursey TE, Cahalan MD, McLaughlin C, Gupta S: Voltage-gated K channels are required for T lymphocyte activation. J Exp Med 160: 369 (1984).PubMedCrossRefGoogle Scholar
  6. 6.
    DeCoursey TE, Chandy KG, Gupta S, Cahalan MD: Voltage-dependent ion channels in T-lymphocytes. J Neuroimmunol 10: 71 (1985).PubMedCrossRefGoogle Scholar
  7. 7.
    Lee SC, Sabath DE, Deutsch C, Prystowsky MB: Increased voltage-gated potassium conductance during interleukin 2-stimulated proliferation of a mouse helper T lymphocyte clone. J Cell Biol 102: 1200 (1986).PubMedCrossRefGoogle Scholar
  8. 8.
    DeCoursey TE, Chandy KG, Gupta S, Cahalan MD: Two types of potassium channels in murine T lymphocytes. J Gen Physiol 89: 379 (1987).PubMedCrossRefGoogle Scholar
  9. 9.
    DeCoursey TE, Chandy KG, Gupta S, Cahalan MD: Mitogen induction of ion channels in murine T lymphocytes. J Gen Physiol 89: 405 (1987).PubMedCrossRefGoogle Scholar
  10. 10.
    Lewis RS and Cahalan MD. Subset-specific expression of potassium channels in developing murine T lymphocytes. Science 239: 771 (1988).PubMedCrossRefGoogle Scholar
  11. 11.
    Chandy KG, DeCoursey TE, Fischbach M, Talal N, Cahalan MD, Gupta S: Altered K+ channel expression in abnormal T lymphocytes from mice with the 1pr gene mutation. Science 233: 1197 (1986).PubMedCrossRefGoogle Scholar
  12. 12.
    Grissmer S, Cahalan MD, Chandy KG: Abundant expression of type I K+ channels: a marker for lymphoproliferative diseases? J Immunol 141: 1137 (1988).PubMedGoogle Scholar
  13. 13.
    Lewis RS and Cahalan MD: The plasticity of ionic channels: parallels between the nervous and immune systems. Trends in Neuroscience 11: 214 (1988).CrossRefGoogle Scholar
  14. 14.
    Choquet D, Sarthou P, Primi D, Cazenave PA and Korn H. Cyclic AMP-modulated potassium channels in murine B cells and their precursors. Science 235: 1211 (1987).PubMedCrossRefGoogle Scholar
  15. 15.
    Sutro JB, Vayuvegula BS, Gupta S and Cahalan MD. Up-regulation of voltage-sensitive K+ channels in mitogen-stimulated B lymphocytes.Biophysical Journal 53: 460a (1988).Google Scholar
  16. 16.
    Vayuvegula B, Gollapudi S, Gupta S: Inhibition of human B cell proliferation by ion channel blockers. In: Gupta S, Paul WE and Fauci AS (Eds.), Mechanisms of Lymphocyte Activation and Immune Regulation. New York: Plenum Press (1987), Advances in Experimental Medicine and Biology 213: 237 (1987).CrossRefGoogle Scholar
  17. 17.
    Lee S, Krause D, Deutsch C: Increased voltage-gated K+ conductance in T-lymphocytes stimulated with phorbol ester. J Physiol 372: 405 (1986).PubMedGoogle Scholar
  18. 19.
    Cahalan MD, Chandy KG, DeCoursey TE, Gupta S: A voltage-gated potassium channel in human T lymphocytes. J Physiol 358: 197 (1985).PubMedGoogle Scholar
  19. 20.
    Sands SB, Lewis RS and Cahalan MD: Charybdotoxin blocks voltage-gated K+ channels in T lymphocytes. Biophys J 53: 260a (1988).Google Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Jeffrey B. Sutro
    • 1
  • Bharathi S. Vayuvegula
    • 2
  • Sudhir Gupta
    • 2
  • Michael D. Cahalan
    • 1
  1. 1.Department of Physiology and Biophysics and Division of Basic and Clinical ImmunologyUniversity of CaliforniaIrvineUSA
  2. 2.Department of MedicineUniversity of CaliforniaIrvineUSA

Personalised recommendations