Flow Cytometric Analysis of Oxidase Activity of Neutrophils from Chronic Granulomatous Disease Patients
Phagocyte cells, neutrophils and macrophages constitute the main defense mechanisms of the human body against invading microorganisms. The bactericidal activity of polymorphonuclear leukocytes (PMNs) involves the activation of a cell membrane associated NADPH oxidase enzyme“. Following activation of the NADPH oxidase enzyme, a cascade of oxidation/reduction reactions occurs, with the generation of several oxygen radicals. Superoxide (0 2 − ) hydrogen peroxide (H202), hydroxyl radical (HO) singlet oxygen (0) are the main oxygen by-products of the respiratory burst of PMNs2 and have an important role in killing of bacteria. The enzyme NADPH oxidase has a major function in the generation of O 2 − radical while other enzymes including superoxide dismutase3, myeloperoxidase4, catalase and glutathione peroxidase5 serve a regulatory activity in the respiratory burst. NADPH oxidase differs from other cellular oxidase enzymes (i.e., mitochondrial), since it is a cyanide insensitive enzyme6. The activation of the NADPH oxidase enzyme with different soluble stimulants as phorbol esters7, formyl-methionyl-leucyl-phenylalanine8 and γ-hexachlorocyclohexane9 results in the generation of active oxygen radicals and mimics the biochemical events which occur during phagocytosis in vivo.
KeywordsNADPH Oxidase Phorbol Myristate Acetate Respiratory Burst Chronic Granulomatous Disease Phorbol Myristate Acetate
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