Legionella Pneumophila Immunity and Immunomodulation: Nature and Mechanisms

  • Herman Friedman
  • Thomas W. Klein
  • Raymond Widen
  • Catherine Newton
  • D. Kay Blanchard
  • Yoshima Yamamoto
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 239)

Abstract

Legionella pneumophila is considered the etiologic agent of legionellosis and may cause severe respiratory infection in susceptible individuals, including man and guinea pigs (1–8). These organisms are fastidious opportunistic pathogens which are ubiquitously found in nature but only occasionally causes disease in man under normal circumstances. Guinea pigs are extremely susceptible to these bacteria and are widely used as an animal model for legionellosis. In contrast, rodents such as mice and rats are highly resistant to infection by these bacteria (7,8,10,11). Mice are difficult to infect with strains of Legionella highly virulent for guinea pigs. In several situations, it has been observed that Legionella may infect immunocompromised individuals, although no particular immunological lesion has been associated with acquisition of the disease (12–14). Indeed, in the first recognized outbreak of legionellosis, i.e., the upper respiratory infection in Pennsylvania State Legionnaires attending a convention in Philadelphia, specific immunologic depression was not observed although individuals who developed the disease drank more liquor, smoked more cigarettes and generally had less sleep than those companions who did not contact the disease.

Keywords

Migration Toxicity Depression Pneumonia Interferon 

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References

  1. 1.
    Fraser, D.W., T.R. Tsai, W. Orenstein, W.E. Parkin, H.J.Beecham, P.G. Sharrar, J. Harris, G.F. Mallison, S.M. Martin, J.E. McDade, C.C. Shepart, P.S. Brachman, and The Field Investigation Team. 1977. Legionnaires’ disease. Description of an epidemic of pneumonia. N. Engl. J. Med. 297: 1198.Google Scholar
  2. 2.
    Meyer, R.P. and S.M. Finegold. 1980. Legionnaires’ disease. Ann. Rev. Med. 31: 219.Google Scholar
  3. 3.
    Fraser, D.W., C. Bopp, I.K. Wachsmuth, J.C. Feeley, and T. Tsai. 1978. Antibiotic treatment of guinea pigs infected with agent of Legionnaires’ disease. Lancet. 1: 175.PubMedCrossRefGoogle Scholar
  4. 4.
    Chandler, F.W., J.E. McDade, M.D. Hicklin, J.A. Blackmon, B.M. Thomason, and E.P. Ewing. 1979. Pathologic findings in guinea pigs inoculated intraperitoneally with the Legionnaires’ disease bacterium. Ann. Inter. Med. 90: 671.Google Scholar
  5. 5.
    Berendt, R.F., H.W. Young, R.G. allen, and G.L. Knutsen. 1980. Dose-response of guinea pigs experimentally infected with aerosols of Legionella pneumophila J. Infect. Dis. 141: 186.Google Scholar
  6. 6.
    Katz, S.M., and S. Hashemi. 1982. Electron microscopic examination of the inflammatory response to Legionella pneumophila in guinea pigs. Lab. Invest. 46: 24.Google Scholar
  7. 7.
    Winn, W.C., Jr., G.S. Davis, D.W. Gump, J.E. Craighead, and H.N. Beaty. 1982. Legionnaires’ pneumonia after intratracheal inoculation of guinea pigs and rats. Lab. Invest. 47: 568.Google Scholar
  8. 8.
    Davis, G.S., W.C. Winn, Jr., D.W. Gump, J.E. Craighead and H.N. Beaty. 1982. Legionnaires’ pneumonia after aerosols exposure of guinea pigs and rats. Am. Rev. Respir. Dis. 126: 1050.Google Scholar
  9. 9.
    Hedlund, K.W., V.G. McGann, D.S. Copeland, S.F. Little and R.G. Allen. 1979. Immunological protection against the Legionnaires’ disease bacterium in the AKR/J mouse. Ann. Intern. Med. 90: 676–679.Google Scholar
  10. 10.
    Rolstadt, B. and P.B. Berdal. 1981. Immune defenses against Legionella pneumophila in rats. Inf. and Immun. 32: 805–812.Google Scholar
  11. 11.
    Eisenstein, T.K. and H. Friedman. 1985. Immunity to Legionella in Legionellosis. 2nd ed., p. 159–169, CRC Press, Boca Raton, Florida.Google Scholar
  12. 12.
    Saravolatz, L.D., R.H. Burch, E. Fisher, T. Madhavan, D. Kiani, T. Neblett and E.L. Quinn. 1979. The compromised host and Legionnaires’ disease. Ann. Intern. Med. 90: 533.Google Scholar
  13. 13.
    Cutz, E., P.S. Thorner, P. Rao, S. Toma, R. Gold and E.W. Gelfand. 1982. Desseminated Legionella pneumophila infection in an infant with severe combined immunodeficiency.Google Scholar
  14. 14.
    Saravolatz, L.D., R.H. Burch, E. Fisher, T. Madhavan, D. Kiani, T. Neblett and E.L. Quinn. 1979. The compromised host and Legionnaires’ disease. Ann. of Inter. Med. 90: 533–537.Google Scholar
  15. 15.
    Arko, R.J., K.H. Wong and J. Feeley. 1979. Immunologic factors affecting the in vivo and in vitro survival of the Legionnaires’ disease bacterium. Ann. Inter. Med. 90: 630–683.Google Scholar
  16. 16.
    Friedman, A.P. and S.M. Katz. 1981. The prevalence of serum antibodies to Legionella pneumophila in patients with chronic pulmonary disease. Am. Rev. Respir. Dis. 123: 238–239.Google Scholar
  17. 17.
    Davis, G.S., W.C. Winn and H.N. Beaty. 1981. Legionnaires’ disease: Infections caused by Legionella pneumophila and Legionella-like organisms. Clin. Chest. Med. 2: 145–66.Google Scholar
  18. 18.
    Winn, W.C.,Jr. and R.L. Myerowitz. 1981. The pathology of the Legionella pneumonias. A review of 74 cases and the literature. Human Pathol. 12: 401–422.CrossRefGoogle Scholar
  19. 19.
    Winn, W. 1984. Pathology and pathogenesis of Legionella infections. In: Legionella, (C.Thornsberry, A. Ballows, J.C. Feeley and W. Jakubowski, Eds.). p. 115–122. Amer. Soc. Microbiol. Press, Washington, D.C.Google Scholar
  20. 20.
    Berendt, R.F., H.W. Young, R.G. Allen et al. 1980. Dose response of guinea pigs experimentally infected with aerosols of Legionella pneumophila. J. Infect. Dis. 141: 186–192.Google Scholar
  21. 21.
    Broome, C.V., W.B. Cherry, W.C. Winn,Jr. and B.R. MacPherson. 1979. Rapid diagnosis of Legionnaires’ disease by direct immunofluorescent staining. Ann. Inter. Med. 90: 1–4.Google Scholar
  22. 22.
    Edson, D.C., H.E. Stiefel, B.B. Wentworth and D.D. Wilson. 1979. Prevalence of antibodies to Legionnaires’ disease. A seroepidemiologic survey of Michigan residents using the hemagglutination test. Ann. Inter. Med. 90: 691–694.Google Scholar
  23. 23.
    Holliday, M.G. 1980. The diagnosis of Legionnaires’ diesease by counterimmunoelectrophoresis. J. Clin. Pathol. 33: 1174–1181.Google Scholar
  24. 24.
    Horwitz, M.A. 1982. Demonstration of cell-mediated immunity in Legionnaires’ disease. Clin. Res. 30: 369A.Google Scholar
  25. 25.
    Wilkinson, H.W., B.J. Fikes and D.D. Cruce. 1979. Indirect immunofluorescence test for serodiagnosis of Leginnaires disease: evidence for serogroup diversity of Legionnaires’ disease bacterial antigens and for multiple specificity of human antibodies. J. Clin. Microbiol. 9: 379–383.Google Scholar
  26. 26.
    Wilkinson, H.W., A.L. Reingold, B.J. Brake, D.L. McGiboney, G.W. Gorman and C.V. Broome. 1983. Reactivity of serum from patients with suspected legionellosis against 29 immunofluorescence assay. J. Infect. Dis. 147: 23–31.Google Scholar
  27. 27.
    Elliot, J.A., W. Johnson and C.M. Helms. 1981. Ultrastructural localization and protective activity of high-molecular-weight antigen isolated from Legionella pneumophila. Infec. Immun. 31: 822–824.Google Scholar
  28. 28.
    Johnson, W., J.A. Elliott, C.M. Helms and E.D. Renner. 1979. A high molecular weight antigen in Legionnaires’ disease bacterium: isolation and partial characterization. Ann. In. Med. 90: 638–641.Google Scholar
  29. 29.
    Elliott, J.A. and W. Johnson. 1981. Immunological and biochemical relationships among flagella isolated from Legionella pneumophila serogroups 1, 2, and 3. Inf. and Immun. 33: 602–609.Google Scholar
  30. 30.
    Friedman, H., R. Widen, T. Klein and W. Johnson. 198. Legionella pneumophila-induced immunostimulation and blastogenesis of normal mouse spleen cells in vitro. Proc. Soc. Exp. Biol. Med. 174: 212–216.Google Scholar
  31. 31.
    Friedman, H., R. Widen, T. Klein and W. Johnson. 1984. Immunostimulation by Legionella pneumophila antigen preparations in vivo and in vitro. Infec. Immun. 43: 347–352.Google Scholar
  32. 32.
    Friedman, H., C. Newton, K.D. Blanchard, T.W. Klein, R. Widen and K.H. Wong. 1986. Immunogenicity and adjuvanticity of lipopolysaccharide from Legionella pneumophila. Proc. Soc. Exp. Biol. Med. (in press).Google Scholar
  33. 33.
    Vogel, F.R., T.W. Klein, S. Specter, M. Hitchings and H. Friedman. 1981. Detection of antibodies for Legionella pneumophila in immune guinea pig serum by solid phase immunofluorescence. J. Clin. Microbiol. 13: 726–729.Google Scholar
  34. 34.
    Friedman, H., R. Widen and T. Klein. 1983. Cellular immunity to Legionella antigen. Clin. Immunol. Newsletter, 4: 92–96.Google Scholar
  35. 35.
    Friedman, H., T. Klein and R. Widen. 1983. Cellular and humoral immunity to Legionella pneumophila. In: Infect. Colloquium 1: p. 99–111. C. Krasemann, ed. W. deGruytes Press, Amsterdam, The Netherlands.Google Scholar
  36. 36.
    Friedman, H., R. Widen, and T. Klein. 1983. Immunodiagnostic assays for legionellosis. Lab. Mgmt. 21: 19–24.Google Scholar
  37. 37.
    Wong, K.H., P.R.B. McMaster, J.C. Feeley, R.J. Arko, W.O. Schalla and F.W. Chandler. 1980. Detection of hypersensitivity to Legionella pneumophila in guinea pigs by skin test. Cur. Microbiol. 4: 105–110.Google Scholar
  38. 38.
    Klein, T., H. Friedman and R. Widen. 1984. The relative potency of virulent vs avirulent Legionella pneumophila for the induction of cell-mediated immunity. Infec. Immun. (in press).Google Scholar
  39. 39.
    Plouffe, J.F. and I.M. Bair. 1981. Lymphocyte transformation to Legionella pneumophila. J. Clin. Lab. Immunol. 5: 149–152.Google Scholar
  40. 40.
    Plouffe, J.F. and I.M. Baird. 1982. Lymphocyte blastogenic responses to L. pneumophila in acute legionellosis. J. Clin. L.b. Immunol. 7: 43: 44.Google Scholar
  41. 41.
    Friedman, H., T. Klein and R. Widen. 1984. Immunity to Legionella pneumophila. In: Legionella p. 145–152. J.C. Feeley, C. Thornsbery, A. Balows and W. Jakubowski. eds. Amer. Soc. Microbiol. Washington, D.C.Google Scholar
  42. 42.
    T. Klein, R. Widen, K. Cabrian, L. Searles and H. Friedman. 1984. Development of cellular immunity in guinea pigs exposed to Legionella pneumophila. In: Legionella p. 178–180. J.C. Feeley, C. Thornsberry, A. Balows and W. Jakubowski, Eds. Amer. Soc. Microbiol., Washington, D.C.Google Scholar
  43. 43.
    Widen, R., I. Lee, T. Klein and H. Friedman. 1983. Blastogenic responsiveness of spleen cells from guinea pigs sensitized to Legionella pneumophila antigen. Proc. Soc. Exp. Biol. Med., 143: 547–544.CrossRefGoogle Scholar
  44. 44.
    Friedman, H., R. Widen, I. Lee and T. Klein. 1983. Cellular immunity to Legionella pneumophila in guinea pigs assessed by direct and indirect migration inhibition reactions in vitro. Infec. Immun. 41: 1132–1137.Google Scholar
  45. 45.
    Friedman, H., R. Widen, T. Klein, L. Searles and K. Cabrian. 1984. Legionella pneumophila-induced blastogenesis of murine lymphoid cells in vitro. Infect. Immun. 43: 314–319.Google Scholar
  46. 46.
    Friedman, H., C. Newton, A. Friedman, R. Widen, T. Klein. 1986. Blastogenic responsiveness of spleen cells from Legionella pneumphila-infected mice immunosuppressed with cyclophosphamide. Current Microbiol. 14: 35–39.CrossRefGoogle Scholar
  47. 47.
    H. Friedman, R.Widen, C. Newton and T. Klein. 1986. Distinctive cytokinetics of blastogenic responses by spleen cells from Legionella pneumophila sensitized mice. Current Microbiol. (in press).Google Scholar
  48. 48.
    Widen, R., C. Newton, T.W. Klein, and H. Friedman. 1985. Interleukin induction by Legionellia pneumophila antigens. Proc. Am. Soc. Mibriol. Ann. Mtg. p. 82.Google Scholar
  49. 49.
    Blanchard, D.K., T.W. Klein, H. Friedman and W.E. Stewart II. 1985. Kinetics and characterization of interferon induction by murine spleen cells stimulated with Legionella pneumophila antigen. Infec. Immun. 49: 719–723.Google Scholar
  50. 50.
    Horwitz, M.A. and S.C. Silverstein. 1981. Interaction of the Legionnaires’ disease bacterium (Legionella pneumophila) with human phagocytes. I. J. Exp. Med. 153: 386–397.Google Scholar
  51. 51.
    Horwitz, M.A. and S. C. Silverstein. 1981. Interaction of the Legionnaires’ disease bacterium (Legionella pneumophila) with human phagocytes. H. J. Exp. Med. 153: 398–406.Google Scholar
  52. 52.
    Horwitz, M.A. 1984. Interactions between Legionella pneumophila and human mononuclear phagocytes. p. 159–166. In: Legionella C. Thornsberry, A. Balows, J.C. Feeley and W. Jakubowski, eds. Amer. Soc. Microbiol. Press. Washington, D.C.Google Scholar
  53. 53.
    Kishimoto, R.A., M.D. Kastello, J.D. White, F.G. Shirey, V.G. McGann, E.W. Larson and K.W. Hedlund. 1979. In vitro interaction between normal cynomolgus monkey alveolar macrophages and Legionnaires’ disease bacteria. Infec. Immun. 25: 761–763.Google Scholar
  54. 54.
    Kishimoto, R.A., J.D. White, F.G. Shirey, V.G. McGann, R.F. Berendt, E.W. Larson and K.W. Hedlund. 1981. In vitro response of guinea pig peritoneal macrophages to Legionella pneumophila. Inf. and Immun. 31: 1209–1213.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Herman Friedman
    • 1
  • Thomas W. Klein
    • 1
  • Raymond Widen
    • 1
  • Catherine Newton
    • 1
  • D. Kay Blanchard
    • 1
  • Yoshima Yamamoto
    • 1
  1. 1.Department of Medical Microbiology and ImmunologyUniversity of South Florida College of MedicineTampaUSA

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