In Vitro Production of Colony-Stimulating Factors by Listeria-Immune Spleen Cells
The survival of mice during Listeria monocytogenes infection is dependent on the efficiency of the early phases of host defenses. These early host defenses consist primarily of the production of bone marrow-derived monocytes and granulocytes and their accumulation at the site of infection. Two important parameters that are believed to affect the production of leukocytes during early infection are the number of leukocyte progenitors in bone marrow and the secretion of colony-stimulating factors (CSFs). At least four discrete CSFs, as determined by an in vitro colony forming assay, have been described: macrophage CSF (M-CSF), granulocyte CSF (G-CSF), granulocyte-macrophage CSF (GM-CSF), and multi-CSF (IL-3) (1). CSFs not only help to regulate the production of phagocytic cells, but also affect the function of mature phagocytes. For example, macrophages stimulated by CSFs secrete increased levels of oxygen reduction products (2) and have increased anti-tumor activity (3,4).
KeywordsListeria Monocytogenes Muramyl Dipeptide Nylon Wool Listeria Monocytogenes Infection Nylon Wool Column
Unable to display preview. Download preview PDF.
- 5.E.J. Wing, A. Waheed, and R.K. Shadduck, Changes in serum colony-stimulating factor and monocyte progenitor cells during Listeria monocytogenes infection in mice, Infect. Immun. 45: 180 (1984).Google Scholar
- 6.E.J. Wing, L. C. Barczynski, A. Waheed, and R.K. Shadduck, Effect of Listeria monocytogenes infection on serum levels of colony-stimulating factor and number of progenitor cells in immune and nonimmmune mice, Infect. Immun. 49: 325 (1985).Google Scholar
- 7.P.R. Wood, A.M. Young, J.L. McKimm-Breschkin, and C. Cheers, Effect of splenectomy on the production of interferon and colony-stimulating factor in Listeria monocytogenes infected mice, Infect. Immun. 46: 860 (1984).Google Scholar
- 8.A.C. Weinblatt, S.N. Vogel, and D.L. Rosenstreich, Adherence to nylon-or rayon-wool columns, in “Manual of macrophage methodology: collection, characterization, and function,” H.B. Herscowitz, H.T. Holden, J.A. Bellanti, and A. Ghaffar eds., Marcel Dekker, New York (1981).Google Scholar
- 11.A. Gallelli, P. Lefrancier, and L. Chedid, Colony-stimulating activity induced by synthetic muramyl dipeptides: Variation with chemical structure and association with anti-infectious activity, Infect. Immun. 46: 495 (1984).Google Scholar
- 12.E.J. Wing, and R.K. Shadduck, Colony-stimulating factor, in “Biological response modifiers,” P.F. Torrence, ed., Academic Press, New York (1985).Google Scholar
- 13.D. Metcalf, The colony-stimulating factors, in “The hemopoietic colony stimulating factors,” Elsevier, Amsterdam (1984).Google Scholar
- 18.S.H.E. Kaufmann, E. Hug, U. Vath, and I. Muller, Effective protection against Listeria monocytogenes and delayed-type hypersensitivity to listerial antigens depend on cooperation between specific L3T4+ and Lyt 2+ cells, Infect. Immun. 48: 263 (1985).Google Scholar