Human Non-Interferon Macrophage Activating Factor: Biological and Biochemical Characterization
A HTLV-1 transformed T cell line has been demonstrated to constitutively produce and secrete a lymphokine with macrophage activating properties. This lymphokine was biologically and biochemically distinct from interferon gamma, the interleukins, the colony stimulating factors and lymphotoxin. In vitro treatment of human monocytes with the partially purified lymphokine resulted in the acquisition of microbicidal and tumoricidal activities. The activity was associated with a protein with an apparent molecular weight of 55,000 daltons and an isoelectric point of 5.5. The potential for the efficacy of such a lymphokine in activating macrophages in vivo provides aspirations for its use in the therapy/prevention of opportunistic infections in immunocompromised hosts.
KeywordsHuman Monocyte Immunocompromised Host Tumoricidal Activity Constitutive Production Colony Stimulate Activity
Unable to display preview. Download preview PDF.
- 4.R.D. Schreiber, Identification of gamma-interferon as a murine macrophage-activating factor for tumor cytotoxicity, Contemp. Top. Immunobiology 13: 171–198 (1984).Google Scholar
- 5.J. Le, W. Prensky, Y.K. Yip, Z. Chang, T. Hoffman, H.C. Stevenson, I. Balazs, J.R. Sadlik, and J. Vilcek, Activation of human monocyte cytotoxicity by natural and recombinant immune interferon, J. Immunol. 131: 2821–2826 (1984).Google Scholar
- 9.J.C. Lee, A.M. Badger, W.J. Johnson, C.P. Sung, and P. Horan, Human non-gamma interferon macrophage activating factor (MT-2/MAF) elicits multiple and cross-species effects on macrophage activation, Proceedings of the Sixth International Congress of Immunology (1986) (abstract).Google Scholar
- 10.J.C. Lee, L. Rebar, J. Laydon, B. Dalton, and P.L. Simon, Induction of interleukin-1 as an indicator for monocyte activation by macrophage activating factor (MT-2/MAF), Lymphokine Research 6: 61 (1987) (abstract).Google Scholar
- 12.E.S. Kleinerman, R. Zicht, P.S. Sarin, R.C. Gallo, and I.J. Fidler, Constitutive production and release of a lymphokine with macrophage-activating factor activity distinct from gamma-interferon by a human T-cell leukemia virus-positive cell line, Cancer Res. 44: 4470–4475 (1984).PubMedGoogle Scholar
- 14.M.S. Meltzer, M. Gilbreath, C.A. Nacy, and R.D. Schreiber, Macrophage activation factor from EL-4 cells distinct from murine gamma interferon (IFN), Fed. Proc. 44: 1697 (1985) (abstract).Google Scholar
- 15.Y. Buchmuller, W.Y. Weiser, and H.G. Remold, Partial purification and characterization of a human lymphokine which induces anti-leishmanial activity in mouse macrophages, Cell. Immun. 90: 242–250 (1985).Google Scholar
- 18.S. Hinuma, K. Sugamura, K. Tsukamoto, and Y. Hinuma, Constitutive production of phagocytosis inducing factor(s) in a monocyte/macrophage lineage cell line (THP-1) by retrovirus-transformed human T cell lines, Microbiol. Immunol. 28: 935–947 (1984).Google Scholar
- 19.J.B. Margolick, J.L. Ambrus, Jr., D.J. Volkman, and A.S. Fauci, Human T4+ lymphocytes produce a phagocytosis-inducing factor (PIF) distinct from interferon-cc and interferon-y, J. Immunol. 136: 546–554 (1986).Google Scholar
- 20.K.H. Grabstein, D.L. Urdal, R.J. Tushinshi, D.Y. Mochizuki, V.L. Price, M.A. Cantrell, S. Gillis, and P.J. Conlon, Induction of macrophage tumoricidal activity by granulocyte-macrophage colony-stimulating factor, Science 232–506 (1986).Google Scholar