Effect of Interferons on the Activation of Murine Peritoneal Macrophages to Inhibit the Growth of Listeria Monocytogenes
Peritoneal macrophages are activated to become antimicrobial in vivo by infection with intracellular parasites (1,2) or in vitro by lymphokine-containing lymphocyte supernatants (3–7). One component of these mitogen stimulated lymphocyte supernatants, gamma interferon (IFN-γ), has been shown to be responsible for the ability of lymphokine supernatants to activate a variety of macrophage functions, including microbicidal and tumoricidal activity. However, there do appear to be non-interferon components of these supernatants that can render macrophages to become microbicidal (8). Izadkhah et al. (9) first demonstrated that treatment of mice with sera containing IFN-γ was able to increase the survival of mice infected with Salmonella typhimurium. More recent studies with pure and recombinant murine IFN-γ (rMuIFN-γ) have demonstrated that this lymphokine can indeed activate macrophages to inhibit the growth of (or kill) several intracellular parasites. IFN-γ treatment (both in vitro and in vivo) inhibits the intracellular protozoa Toxoplasma gondii (10,11) and Leishmania donovani (12) and the in vivo growth of Listera monocytogenes (13). A recent report indicates that the in vivo production of IFN-γ is required for the clearance of Listeria in infected mice (14). In human studies IFN-γ has been shown to activate peripheral monocytes to kill Toxoplasma gondii (15) and Leishmania donovani in vitro (12, 16) and a recent study describes the enhanced capacity of these cells to kill Listeria (17).
KeywordsPeritoneal Macrophage Gamma Interferon Listeria Monocytogenes Mouse Peritoneal Macrophage Peritoneal Exudate Cell
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- 7.J.L. Krahenbuhl, and J.S. Remington. In vitro induction of nonspecific resistance in macrophages by specifically sensitized lymphocytes. Infect. Immun. 4: 337–343 (1971).Google Scholar
- 8.C.A. Nacy, A.H. Fortier, M.S. Meltzer, N.A. Buchmeier, and R.D. Schreiber, Macrophage activation to kill Leishmania major: activation of macrophages for intracellular destruction of amastigotes can be induced by both recombinant interferon-Y and non-interferon lymphokines. J. Immunol. 135: 3505–3511 (1985).PubMedGoogle Scholar
- 22.A.M. Badger, S.R. Cooperband, and V.J. Merluzzi, The production of proliferation inhibitory factor (PIF) by mouse spleen cells in vitro. J.Immunol. 112: 1435–1443 (1974).Google Scholar
- 23.L., Harrington-Fowler, P.M. Henson, and M.S. Wilder, Fate of Listeria monocytogenes in resident and activated macrophages. Inf. and Immun. 33: 11–16 (1981).Google Scholar
- 24.G.L. Spitalny, and R.J. North, Destruction of Listeria monocytogenes in vitro. In: Methods for Studying Mononuclear phagocytes. Ed. Adams, D.O., Edelson, P.J. and Hillel, K. Academic Press. 685–691 (1981).Google Scholar
- 25.J.J. Mond, F.D. Finkelman, C. Sarma, J. Ohara, and S. Serrate, Recombinant interferon-Y inhibits the B cell proliferative response stimulated by soluble but not by sepharose-bound anti-immunoglobulin antibody. J. Immunol. 135: 2513–2517 (1983).Google Scholar
- 31.P.A. Campbell, Are inflammatory phagocytes responsible for resistance to facultative intracellular bacteria Immunol. Today 7 (3): 70–72 (1986).Google Scholar
- 32.R.C. Bast, Jr., R.P. Cleveland, B.H. Littman, B. Zbar, and H.J. Rapp, Acquired cellular immunity: extracellular killing of Listeria monocytogenes by a product of immunologically activated macrophages. Cell. Immunol. 10: 248–259 (1974).Google Scholar
- 34.K.K. Sethi, M. Teschner, and H. Brandis, In vitro antilisterial activity of soluble product(s) released from Listeria-immune murine peritoneal macrophages. Infect.Immun. 10:960–962. (1974).Google Scholar
- 35.D. Adam, F. Staber, B.H.,Belohradsky, and W. Marget, Effect of dihydrostreptomycin on phagocytosis of mouse peritoneal macrophages in vitro. Infect. Immun. 5: 537–541 (1972).Google Scholar
- 40.J. Bennedsen, S. J. Riisgaard, M. Rhodes, and S.O. Larsen, In vitro studies on normal stimulated and immunologically activated mouse macrophages III. Intracellular multiplication of Listeria monocytogenes. Acta Pathol. Microbiol. Scand.Sect.C 85: 246–252 (1977).Google Scholar