Advertisement

Objective Lenses for Confocal Microscopy

  • H. Ernst Keller

Abstract

No other component of the microscope is as instrumental in determining the information content of an image as the objective. The resolved detail, the contrast at which this detail is presented, the depth through the object from which useful information can be derived and the diameter of the useful field are all limited by the performance of the objective. All other imaging components, such as relay optics, Telan systems, tube lenses and eyepieces, or pro-jectives may have some corrective function but otherwise serve only to present the image generated by the objective to the detector in such a way that most of its information content can be recorded without degradation.

Keywords

Numerical Aperture Confocal Scanning Multilayer Coating Spherical Aberration Chromatic Aberration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Inoué, S., and Oldenbourg, R., 1993, Optical instruments: Microscopes. In: Handbook of Optics (Optical Society of America, ed), 2nded. McGraw-Hill, New York.Google Scholar
  2. Maly, M., and Boy de, A., 1994, Real-time steroscopic confocal reflection confocal microscopy using objective lenses with linear longitudinal chromatic dispersion, Scanning 16:187–193.Google Scholar
  3. Pawley, J.B., Amos, W.B., Dixon, A., Brelje, T.C., 1993, Simultaneous, non-interfering collection of optimal fluorescent andbackscattered light signals on the MRC 500/600. In: Proceedings of the Fifty-first Annual Meeting of the Microscopy Society of America, San Francisco Press, Inc., San Francisco, pp. 156–157.Google Scholar
  4. Petroll, W.M., Cavanaugh, H.D., and Jester, J.V., 1993, Three dimensional imaging of corneal cells using in vivo vconfocal microscopy, J. Microsc. 170:213–219.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • H. Ernst Keller
    • 1
  1. 1.Carl Zeiss, Inc.ThornwoodUSA

Personalised recommendations