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Laser Sources for Confocal Microscopy

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Handbook of Biological Confocal Microscopy

Abstract

In this chapter we describe the characteristic properties of a number of lasers commonly used in fluorescence microscopy. We concentrate on the characteristics of lasers in relation to their use as an illumination source. Lasers have a number of unique properties compared to other sources emitting electromagnetic radiation, such as arc lamps, which make them an almost ideal light source for use in confocal microscopy. These properties are:

  • High degree of monochromaticity

  • Small divergence

  • High brightness

  • High degree of spatial and temporal coherence

  • Plane polarized emission (for many types)

  • A Gaussian beam profile (can be obtained by special optics)

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Authors and Affiliations

  1. Department of Physics, Laboratory for Fluorescence Dynamics, University of Illinois at Urbana-Champaign, 1110 W. Green St., Urbana, Illinois, 61801, USA

    Enrico Gratton

  2. ISS Inc., P.O. Box 6122, Champaign, Illinois, 61821, USA

    Martin J. vandeVen

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  1. Enrico Gratton
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  2. Martin J. vandeVen
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Editors and Affiliations

  1. Zoology Department, University of Wisconsin-Madison, Madison, Wisconsin, USA

    James B. Pawley

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Gratton, E., vandeVen, M.J. (1995). Laser Sources for Confocal Microscopy. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5348-6_5

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  • DOI: https://doi.org/10.1007/978-1-4757-5348-6_5

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