The Pixilated Image

  • Robert H. Webb
  • C. Kathleen Dorey


There are optimal settings of magnification and detector gain for confocal microscopes. This chapter will show that the “zoom” magnification control must be chosen to produce a sampling rate that conforms to the Nyquist criterion so that we don’t lose or confuse the data by undersampling and so that we don’t “cook” the sample by oversampling. The material described here is generally applicable to all imaging systems, but is of particular import for the flexible digital images available from a confocal laser scanning microscope (CLSM).


Gray Level Automatic Gain Control Bright Point Optical Resolution Axial Resolution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Robert H. Webb
    • 1
  • C. Kathleen Dorey
    • 1
  1. 1.Schepens Eye Research Institute and MGH Laser CenterBostonUSA

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