Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen

  • Victoria Centonze
  • James Pawley


The other chapters in this book give the reader an in-depth description of almost every important aspect of biological confocal microscopy that we could think of. This chapter is to provide the novice user of this instrument with a basic understanding of the practical information needed to use it effectively. Because the computer interfaces of the various commercial instruments vary greatly, this chapter will stress the important features of microscopical optics and the basics of sampling that are common to all instruments. The underlying agenda of these suggestions is based on two principles:
  • Don’t waste photons.

  • Get all of the optical performance that you have paid for.


Focus Plane Chromatic Aberration Cleaning Solution Lens Tissue Aperture Plane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Victoria Centonze
    • 1
  • James Pawley
    • 2
  1. 1.IMR, Animal Science BuildingUniversity of WisconsinMadisonUSA
  2. 2.Zoology DepartmentUniversity of WisconsinMadisonUSA

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