Abstract
Three-dimensional (3D) microscopy requires the collection of data over a certain volume in the specimen, followed by a suitable two-dimensional (2D) visualization procedure in which the desired image is produced. In holography, nature offers us a potential pathway by which the 3D structure of an object can be observed directly through wavefront reconstruction techniques. However, the slow time response of holographic recording media prevents real-time applications of this technique. In addition, while in principle this approach might work in reflection, holographic fluorescence images are impossible because emitted fluorescence radiation is incoherent.
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References
Agard, D.A., Hiraoka, Y., Shaw, P., and Sedat, J.W., 1989, Microscopy in three dimensions, Methods Cell Biol. 30:353–377.
Brakenhoff, G.J., 1979, Imaging modes in confocal scanning light microscopy (CSLM), J.Microsc. 117(2):233–242.
Brakenhoff, G.J., and van der Voort, H.T.M., 1989, Illumination and detection strategies for confocal microscopy, Proc. SPIE 1139:117–120.
Brakenhoff, G.J., and Visscher, K., 1992, Confocal imaging with bilateral scanning and array detectors, J. Microsc. 165:139–146.
Brakenhoff, G.J., and Visscher, K., 1993, Imaging modes for bilateral confocal scanning microscopy, J. Microsc. 171:17–26.
Denk, W., Strickler, J.H., and Webb, W.W., 1990, Two-photon laser scanning fluorescence microscopy, Science 248:73–76.
Pawley, J.B., 1994, The sources of noise in three-dimensional microscopical data sets. In: Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens (J. Stevens, ed.), University of Toronto, Academic Press. San Diego.
Petráň, M., and Hadravsky, M., 1968, Tandem-scanning reflected light microscope, J. Opt. Soc, 58:661–664.
Sheppard, C.J.R., 1992, Signal-to-noise in confocal microscope systems, J. Microsc. 168:209–218.
Tsien, R.Y., and Harvotrinian, A.T., 1990, Practical design criteria for a dynamic ratio imaging system, Cell Calcium 11:93–109.
Tsien, R.Y., and Waggoner, A., 1989, Fluorophores for confocal microscopy: Photophysics and photochemistry. In: Handbook of Biological Confocal Microscopy (J.B. Pawley, ed.), Plenum Press, New York.
van den Engh, G., and Farmer, C., 1992, Photo-bleaching and photon saturation in flow cytometry, Cytometry 13:669–677.
van der Voort, H.T.M., Brakenhoff, G.J., and Baarslag, M.W., 1989, Three-dimensional visualization methods for confocal microscopy, J. Microsc. 153:123–132.
Wells, K.S., Sandison, D.R., Strickler, J., and Webb, W.W., 1989, Quantitative fluorescence imaging with laser scanning confocal microscopy. In: Handbook of Biological Confocal Microscopy (J. Pawley, ed.), Plenum Press, London, pp. 27–39.
White J.G., 1991, Confocal imaging system, U.S. Patent No. 5.032,720.
Xiao, G.Q., and Kino, G.S., 1987, A real-time confocal scanning optical microscope, Proc. SPIE 809:107–113.
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© 1995 Springer Science+Business Media New York
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Brakenhoff, G.J., Visscher, K. (1995). Real-Time Stereo (3D) Confocal Microscopy. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5348-6_21
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DOI: https://doi.org/10.1007/978-1-4757-5348-6_21
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