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Real-Time Stereo (3D) Confocal Microscopy

  • G. J. Brakenhoff
  • K. Visscher

Abstract

Three-dimensional (3D) microscopy requires the collection of data over a certain volume in the specimen, followed by a suitable two-dimensional (2D) visualization procedure in which the desired image is produced. In holography, nature offers us a potential pathway by which the 3D structure of an object can be observed directly through wavefront reconstruction techniques. However, the slow time response of holographic recording media prevents real-time applications of this technique. In addition, while in principle this approach might work in reflection, holographic fluorescence images are impossible because emitted fluorescence radiation is incoherent.

Keywords

Confocal Image Emission Flux Emission Capability Readout Noise Camera Plane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • G. J. Brakenhoff
    • 1
  • K. Visscher
    • 1
  1. 1.Section of Molecular Cytology, Institute for Molecular Cell Biology, BioCentrum AmsterdamUniversity of AmsterdamAmsterdamThe Netherlands

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