Primary Neural Cell Cultures and GM1 Monosialoganglioside: A Model for Comprehension of the Mechanisms Underlying GM1 Effects in CNS Repair Process in Vivo
Contrary to long-held pessimistic notions, it is now recognized that not only the developing but also the adult mammalian central nervous system (C.N.S.) is capable of undergoing a series of repair processes following injury. Although the irreversibly damaged neurons cannot be replaced, repair in the adult can partly be accomplished by additional growth and reorganization of neuronal processes from undamaged axons in response to adjacent axonal and synaptic degeneration. The intensity of this process varies with the extent, localization and type of neuronal injury and is presumably determined by appropriate signalling, i.e. neuronotrophic factors, from humoral surroundings, cell-cell contacts and denervated target areas. In at least some situations, a close correlation between lesion-induced remodeling and behavioral recovery has been reported (Nieto-Sampedro et al., 1983). Agents or conditions capable of enhancing the availability or activity of neuronotrophic factors are currently being examined in the attempt to ameliorate functional recovery of damaged CNS neuronal tissue.
KeywordsGaba Uptake Adult Mammalian Brain Mesencephalic Cell Mesencephalic Dopaminergic Neuron Exogenous Ganglioside
central nervous system
Eagle’ls basal medium
phosphate buffered saline
glial fibrillary protein
gloxylic acid induced fluorescence
dorsal root ganglia
nerve growth factor
platelet derived growth factor.
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